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	<title>bioRxiv Channel: MoTrPAC</title>
	<link>https://biorxiv.org</link>
	<description>
	This feed contains articles for bioRxiv Channel "MoTrPAC"
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	<item rdf:about="https://biorxiv.org/cgi/content/short/2023.01.13.523698v1?rss=1">
<title>
<![CDATA[
The mitochondrial multi-omic response to exercise training across tissues 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2023.01.13.523698v1?rss=1"
</link>
<description><![CDATA[
Mitochondria are adaptable organelles with diverse cellular functions critical to whole-body metabolic homeostasis. While chronic endurance exercise training is known to alter mitochondrial activity, these adaptations have not yet been systematically characterized. Here, the Molecular Transducers of Physical Activity Consortium (MoTrPAC) mapped the longitudinal, multi-omic changes in mitochondrial analytes across 19 tissues in male and female rats endurance trained for 1, 2, 4 or 8 weeks. Training elicited substantial changes in the adrenal gland, brown adipose, colon, heart and skeletal muscle, while we detected mild responses in the brain, lung, small intestine and testes. The colon response was characterized by non-linear dynamics that resulted in upregulation of mitochondrial function that was more prominent in females. Brown adipose and adrenal tissues were characterized by substantial downregulation of mitochondrial pathways. Training induced a previously unrecognized robust upregulation of mitochondrial protein abundance and acetylation in the liver, and a concomitant shift in lipid metabolism. The striated muscles demonstrated a highly coordinated response to increase oxidative capacity, with the majority of changes occurring in protein abundance and post-translational modifications. We identified exercise upregulated networks that are downregulated in human type 2 diabetes and liver cirrhosis. In both cases HSD17B10, a central dehydrogenase in multiple metabolic pathways and mitochondrial tRNA maturation, was the main hub. In summary, we provide a multi-omic, cross-tissue atlas of the mitochondrial response to training and identify candidates for prevention of disease-associated mitochondrial dysfunction.
]]></description>
<dc:creator>Amar, D.</dc:creator>
<dc:creator>Gay, N. R.</dc:creator>
<dc:creator>Jimenez-Morales, D.</dc:creator>
<dc:creator>Jean Beltran, P. M.</dc:creator>
<dc:creator>Ramaker, M. E.</dc:creator>
<dc:creator>Natarajan Raja, A.</dc:creator>
<dc:creator>Zhao, B.</dc:creator>
<dc:creator>Sun, Y.</dc:creator>
<dc:creator>Marwaha, S.</dc:creator>
<dc:creator>Gaul, D.</dc:creator>
<dc:creator>Hershman, S. G.</dc:creator>
<dc:creator>Xia, A.</dc:creator>
<dc:creator>Lanza, I.</dc:creator>
<dc:creator>Fernandez, F. M.</dc:creator>
<dc:creator>Montgomery, S. B.</dc:creator>
<dc:creator>Hevener, A. L.</dc:creator>
<dc:creator>Ashley, E.</dc:creator>
<dc:creator>Walsh, M. J.</dc:creator>
<dc:creator>Sparks, L. M.</dc:creator>
<dc:creator>Burant, C. F.</dc:creator>
<dc:creator>Rector, R. S.</dc:creator>
<dc:creator>Thyfault, J.</dc:creator>
<dc:creator>Wheeler, M. T.</dc:creator>
<dc:creator>Goodpaster, B. H.</dc:creator>
<dc:creator>Coen, P. M.</dc:creator>
<dc:creator>Schenk, S.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:date>2023-01-13</dc:date>
<dc:identifier>doi:10.1101/2023.01.13.523698</dc:identifier>
<dc:title><![CDATA[The mitochondrial multi-omic response to exercise training across tissues]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2023-01-13</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2023.01.10.523450v1?rss=1">
<title>
<![CDATA[
Multiomic identification of key transcriptional regulatory programs during endurance exercise training 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2023.01.10.523450v1?rss=1"
</link>
<description><![CDATA[
Transcription factors (TFs) play a key role in regulating gene expression. We conducted an integrated analysis of chromatin accessibility, DNA methylation, mRNA expression, protein abundance and phosphorylation across eight tissues in fifty rats of equally represented sexes following endurance exercise training (EET) to identify coordinated epigenomic and transcriptional changes and determine key TFs involved. We uncovered tissue-specific EET associated changes and TF motif enrichment across differentially expressed genes (DEGs), accessible regions (DARs), and methylated regions (DMRs). We discovered distinct routes of EET-induced regulation through either epigenomic alterations providing better access for TFs to affect target genes, or via changes in TF expression or activity enabling target gene responses. We identified TF motifs enriched among correlated epigenomic and transcriptomic alterations, DEGs correlated with exercise-related phenotypic and cell type composition changes, and EET-induced activity changes of TFs whose target genes are enriched for DEGs. This analysis elucidates the unique gene regulatory mechanisms mediating diverse transcriptional responses to EET across tissues.
]]></description>
<dc:creator>Smith, G. R.</dc:creator>
<dc:creator>Zhao, B.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Raja, A.</dc:creator>
<dc:creator>Viggars, M. R.</dc:creator>
<dc:creator>Pincas, H.</dc:creator>
<dc:creator>Gay, N. R.</dc:creator>
<dc:creator>Sun, Y.</dc:creator>
<dc:creator>Ge, Y.</dc:creator>
<dc:creator>Nair, V. D.</dc:creator>
<dc:creator>Sanford, J. A.</dc:creator>
<dc:creator>Amper, M. A. S.</dc:creator>
<dc:creator>Vasoya, M.</dc:creator>
<dc:creator>Smith, K. S.</dc:creator>
<dc:creator>Montgomery, S. B.</dc:creator>
<dc:creator>Zaslavsky, E.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Esser, K. A.</dc:creator>
<dc:creator>Walsh, M. J.</dc:creator>
<dc:creator>Snyder, M.</dc:creator>
<dc:creator>Sealfon, S. C.</dc:creator>
<dc:date>2023-01-12</dc:date>
<dc:identifier>doi:10.1101/2023.01.10.523450</dc:identifier>
<dc:title><![CDATA[Multiomic identification of key transcriptional regulatory programs during endurance exercise training]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2023-01-12</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2023.02.03.527012v1?rss=1">
<title>
<![CDATA[
Sexual dimorphism and the multi-omic response to exercise training in rat subcutaneous white adipose tissue 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2023.02.03.527012v1?rss=1"
</link>
<description><![CDATA[
Subcutaneous white adipose tissue (scWAT) is a dynamic storage and secretory organ that regulates systemic homeostasis, yet the impact of endurance exercise training and sex on its molecular landscape has not been fully established. Utilizing an integrative multi-omics approach with data generated by the Molecular Transducers of Physical Activity Consortium (MoTrPAC), we identified profound sexual dimorphism in the dynamic response of rat scWAT to endurance exercise training. Despite similar cardiorespiratory improvements, only male rats reduced whole-body adiposity, scWAT adipocyte size, and total scWAT triglyceride abundance with training. Multi-omic analyses of adipose tissue integrated with phenotypic measures identified sex-specific training responses including enrichment of mTOR signaling in females, while males displayed enhanced mitochondrial ribosome biogenesis and oxidative metabolism. Overall, this study reinforces our understanding that sex impacts scWAT biology and provides a rich resource to interrogate responses of scWAT to endurance training.
]]></description>
<dc:creator>Many, G. M.</dc:creator>
<dc:creator>Sanford, J. A.</dc:creator>
<dc:creator>Sagendorf, T. J.</dc:creator>
<dc:creator>Hou, Z.</dc:creator>
<dc:creator>Nigro, P.</dc:creator>
<dc:creator>Whytock, K. L.</dc:creator>
<dc:creator>Amar, D.</dc:creator>
<dc:creator>Caputo, T.</dc:creator>
<dc:creator>Gay, N. R.</dc:creator>
<dc:creator>Gaul, D. A.</dc:creator>
<dc:creator>Hirshman, M. F.</dc:creator>
<dc:creator>Jimenez-Morales, D.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Muehlbauer, M. J.</dc:creator>
<dc:creator>Vamvini, M.</dc:creator>
<dc:creator>Bergman, B. C.</dc:creator>
<dc:creator>Fernandez, F. M.</dc:creator>
<dc:creator>Goodyear, L. J.</dc:creator>
<dc:creator>Ortlund, E. A.</dc:creator>
<dc:creator>Sparks, L. M.</dc:creator>
<dc:creator>Xia, A.</dc:creator>
<dc:creator>Adkins, J. N.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Newgard, C. B.</dc:creator>
<dc:creator>Schenk, S.</dc:creator>
<dc:creator>Study Group, T. M.</dc:creator>
<dc:date>2023-02-04</dc:date>
<dc:identifier>doi:10.1101/2023.02.03.527012</dc:identifier>
<dc:title><![CDATA[Sexual dimorphism and the multi-omic response to exercise training in rat subcutaneous white adipose tissue]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2023-02-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2022.09.21.508770v1?rss=1">
<title>
<![CDATA[
Temporal dynamics of the multi-omic response to endurance exercise training across tissues 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2022.09.21.508770v1?rss=1"
</link>
<description><![CDATA[
Regular exercise promotes whole-body health and prevents disease, yet the underlying molecular mechanisms throughout a whole organism are incompletely understood. Here, the Molecular Transducers of Physical Activity Consortium (MoTrPAC) profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome, and immunome in whole blood, plasma, and 18 solid tissues in Rattus norvegicus over 8 weeks of endurance exercise training. The resulting data compendium encompasses 9466 assays across 19 tissues, 25 molecular platforms, and 4 training time points in young adult male and female rats. We identified thousands of shared and tissue- and sex-specific molecular alterations. Temporal multi-omic and multi-tissue analyses demonstrated distinct patterns of tissue remodeling, with widespread regulation of immune, metabolism, heat shock stress response, and mitochondrial pathways. These patterns provide biological insights into the adaptive responses to endurance training over time. For example, exercise training induced heart remodeling via altered activity of the Mef2 family of transcription factors and tyrosine kinases. Translational analyses revealed changes that are consistent with human endurance training data and negatively correlated with disease, including increased phospholipids and decreased triacylglycerols in the liver. Sex differences in training adaptation were widespread, including those in the brain, adrenal gland, lung, and adipose tissue. Integrative analyses generated novel hypotheses of disease relevance, including candidate mechanisms that link training adaptation to non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular health, and tissue injury and recovery. The data and analysis results presented in this study will serve as valuable resources for the broader community and are provided in an easily accessible public repository (https://motrpac-data.org/).

HighlightsO_LIMulti-tissue resource identifies 35,439 analytes regulated by endurance exercise training at 5% FDR across 211 combinations of tissues and molecular platforms.
C_LIO_LIInterpretation of systemic and tissue-specific molecular adaptations produced hypotheses to help describe the health benefits induced by exercise.
C_LIO_LIRobust sex-specific responses to endurance exercise training are observed across multiple organs at the molecular level.
C_LIO_LIDeep multi-omic profiling of six tissues defines regulatory signals for tissue adaptation to endurance exercise training.
C_LIO_LIAll data are available in a public repository, and processed data, analysis results, and code to reproduce major analyses are additionally available in convenient R packages.
C_LI
]]></description>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:creator>Amar, D.</dc:creator>
<dc:creator>Gay, N. R.</dc:creator>
<dc:creator>Jean Beltran, P. M.</dc:creator>
<dc:creator>Adkins, J. N.</dc:creator>
<dc:creator>Almagro Armenteros, J. J.</dc:creator>
<dc:creator>Ashley, E.</dc:creator>
<dc:creator>Avila-Pacheco, J.</dc:creator>
<dc:creator>Bae, D.</dc:creator>
<dc:creator>Bararpour, N.</dc:creator>
<dc:creator>Burant, C.</dc:creator>
<dc:creator>Clish, C.</dc:creator>
<dc:creator>Cutter, G.</dc:creator>
<dc:creator>Dasari, S.</dc:creator>
<dc:creator>Dennis, C.</dc:creator>
<dc:creator>Evans, C. R.</dc:creator>
<dc:creator>Fernandez, F. M.</dc:creator>
<dc:creator>Gaul, D.</dc:creator>
<dc:creator>Ge, Y.</dc:creator>
<dc:creator>Gerszten, R.</dc:creator>
<dc:creator>Goodyear, L. J.</dc:creator>
<dc:creator>Hou, Z.</dc:creator>
<dc:creator>Ilkayeva, O.</dc:creator>
<dc:creator>Ivanova, A. A.</dc:creator>
<dc:creator>Jimenez-Morales, D.</dc:creator>
<dc:creator>Kachman, M. T.</dc:creator>
<dc:creator>Keshishian, H.</dc:creator>
<dc:creator>Kraus, W. E.</dc:creator>
<dc:creator>Lanza, I. R.</dc:creator>
<dc:creator>Li, J.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Lira, A. C.</dc:creator>
<dc:creator>Many, G. M.</dc:creator>
<dc:creator>Marwaha, S.</dc:creator>
<dc:creator>Miller, M. E.</dc:creator>
<dc:creator>Muehlbauer, M. J.</dc:creator>
<dc:creator>Nair, K. S.</dc:creator>
<dc:creator>Nair, V. D.</dc:creator>
<dc:creator>Natarajan Raja, A.</dc:creator>
<dc:creator>Newgard, C.</dc:creator>
<dc:creator>Ortlund, E. A.</dc:creator>
<dc:creator>Pie</dc:creator>
<dc:date>2022-09-23</dc:date>
<dc:identifier>doi:10.1101/2022.09.21.508770</dc:identifier>
<dc:title><![CDATA[Temporal dynamics of the multi-omic response to endurance exercise training across tissues]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2022-09-23</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2025.04.10.647997v1?rss=1">
<title>
<![CDATA[
Sexually distinct multi omic responses to progressive endurance exercise training in the rat lung. Findings from MoTrPAC 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2025.04.10.647997v1?rss=1"
</link>
<description><![CDATA[
Despite the lungs being essential for ventilation and aerobic exercise capacity, conventionally the lungs are not thought to adapt to exercise training. Endurance exercise is key to pulmonary rehabilitation programs, which also displays sex-specific differences in therapeutic efficacy. Given the molecular underpinnings of sex-specific lung adaptations to endurance exercise are uncharacterized, we used a multi-omics approach to study sex differences in the lungs of 6-month-old Fischer 344 rats in response to an 8 week progressive endurance treadmill training protocol. This was accomplished by reannotating publicly accessible data from the Molecular Transducers of Physical Activity Consortium (MoTrPAC) and integrating newly-analyzed acetylome data to assess multi-omic sex differences in sedentary and progressively trained states. Female rats displayed enrichment in immune-related features and pathways at the transcriptome and proteome level that were maintained with training. Conversely, in the male rat lung there was an overall decrease in immune pathways following 8 weeks of training. Sexually conserved responses to training included increased enrichment in transcriptomic pathways related to type I alveoli and proteomic pathways related to cilia, and decreased mitochondrial protein acetylation. In both sexes, features known to be enriched in lung diseases were attenuated with training. Together our findings provide novel insight into sex specific responses to endurance exercise training in the rat lung and may offer translational insight into sex-specific differences in lung disease pathogenesis and treatment.
]]></description>
<dc:creator>Many, G.</dc:creator>
<dc:creator>Sagendorf, T. J.</dc:creator>
<dc:creator>Mitchell, H. D.</dc:creator>
<dc:creator>Sanford, J. A.</dc:creator>
<dc:creator>Cohen, S.</dc:creator>
<dc:creator>Misra, R.</dc:creator>
<dc:creator>Estevao, I. L.</dc:creator>
<dc:creator>Diaz Ludovico, I.</dc:creator>
<dc:creator>Gaul, D. A.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Ushakumary, M. G.</dc:creator>
<dc:creator>Pino, J.</dc:creator>
<dc:creator>Musi, N.</dc:creator>
<dc:creator>Nie, J.</dc:creator>
<dc:creator>Fernandez, F.</dc:creator>
<dc:creator>Ortlund, E. A.</dc:creator>
<dc:creator>Esser, K. A.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Schenk, S.</dc:creator>
<dc:creator>Clair, G. C.</dc:creator>
<dc:creator>Adkins, J. N.</dc:creator>
<dc:date>2025-04-16</dc:date>
<dc:identifier>doi:10.1101/2025.04.10.647997</dc:identifier>
<dc:title><![CDATA[Sexually distinct multi omic responses to progressive endurance exercise training in the rat lung. Findings from MoTrPAC]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2025-04-16</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2026.03.03.704460v1?rss=1">
<title>
<![CDATA[
Exercise modulation of the alternative splicing landscape in human tissues 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2026.03.03.704460v1?rss=1"
</link>
<description><![CDATA[
The diverse health benefits of exercise are associated with multi-organ molecular responses. Alternative RNA splicing (AS) is an important determinant of transcriptome and proteome diversity. We profiled the temporal effects of acute endurance and resistance exercise on the AS landscape of human skeletal muscle, adipose tissue, and blood, and studied regulatory mechanisms through integrated multi-omic analyses. We identified 5102 distinct differential AS (DAS) events, with the majority modifying protein-coding sequence (89%) and being independent of altered RNA expression (67%). Endurance and resistance exercise induced differing patterns of AS alterations with divergent temporal trajectories. We inferred the DAS-associated RNA-binding and DNA-binding proteins. In skeletal muscle, where DAS events were the most abundant, DAS genes were enriched for muscle structure- and RNA splicing-related processes, and splicing machinery components were regulated at the protein phosphorylation, RNA, and AS levels. These findings implicate AS regulation as a major mediator of the responses to exercise.
]]></description>
<dc:creator>Zhang, Z.</dc:creator>
<dc:creator>Nudelman, G.</dc:creator>
<dc:creator>Pincas, H.</dc:creator>
<dc:creator>Iyer, G.</dc:creator>
<dc:creator>Smith, G. R.</dc:creator>
<dc:creator>Keshishian, H.</dc:creator>
<dc:creator>Jin, C. A.</dc:creator>
<dc:creator>Trappe, S.</dc:creator>
<dc:creator>Katz, D. H.</dc:creator>
<dc:creator>Burant, C. F.</dc:creator>
<dc:creator>Nair, V. D.</dc:creator>
<dc:creator>Zaslavsky, E.</dc:creator>
<dc:creator>Sealfon, S. C.</dc:creator>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:date>2026-03-04</dc:date>
<dc:identifier>doi:10.64898/2026.03.03.704460</dc:identifier>
<dc:title><![CDATA[Exercise modulation of the alternative splicing landscape in human tissues]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2026-03-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2026.03.02.705347v1?rss=1">
<title>
<![CDATA[
Molecular Transducers of Physical Activity Consortium (MoTrPAC): Initial Insights into the Dynamic Human Responses to Exercise 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2026.03.02.705347v1?rss=1"
</link>
<description><![CDATA[
The goal of the Molecular Transducers of Physical Activity Consortium (MoTrPAC) is to examine the physiological and molecular basis for health benefits in response to acute and chronic exercise. Prior to COVID-19 suspension, healthy, sedentary participants (N=206, 18-74y) were randomized to endurance exercise (N=80), resistance exercise (N=81), or non-exercise control (N=45) interventions. The prescribed vigorous acute endurance and resistance exercise bouts induced physiological and metabolic perturbations relative to resting homeostasis. The supervised chronic (3d/wk, 12wk) endurance or resistance training programs robustly improved several physiological parameters (i.e., VO2peak, muscular strength). Temporal biospecimen (blood, muscle, and adipose) collections and processing coupled to the acute exercise bouts were highly successful. In most cases, over 90% success was achieved for blood, muscle, and adipose samples. Endurance and resistance exercise induced distinct acute and chronic physiological responses, which provide a framework to interrogate the molecular basis for health adaptations to these two popular exercise modalities.



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]]></description>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:creator>Brandt, A. R.</dc:creator>
<dc:creator>Fleg, J.</dc:creator>
<dc:creator>Goodpaster, B. H.</dc:creator>
<dc:creator>Jaeger, B.</dc:creator>
<dc:creator>Jin, C. A.</dc:creator>
<dc:creator>Johannsen, N. M.</dc:creator>
<dc:creator>Katz, D.</dc:creator>
<dc:creator>Keshishian, H.</dc:creator>
<dc:creator>Kohrt, W. M.</dc:creator>
<dc:creator>Kraus, W. E.</dc:creator>
<dc:creator>Lester, B.</dc:creator>
<dc:creator>Melanson, E. L.</dc:creator>
<dc:creator>Miller, M. E.</dc:creator>
<dc:creator>Montalvo, S.</dc:creator>
<dc:creator>Rejeski, W. J.</dc:creator>
<dc:creator>Shimly, S. M.</dc:creator>
<dc:creator>Smith, G. R.</dc:creator>
<dc:creator>Stowe, C. L.</dc:creator>
<dc:creator>Trappe, S.</dc:creator>
<dc:creator>AbouAssi, H.</dc:creator>
<dc:creator>Adams, N.</dc:creator>
<dc:creator>Amar, D.</dc:creator>
<dc:creator>Ashley, E.</dc:creator>
<dc:creator>Aslamy, A.</dc:creator>
<dc:creator>Bamman, M. M.</dc:creator>
<dc:creator>Belangee, A.</dc:creator>
<dc:creator>Bennett, W.</dc:creator>
<dc:creator>Bergman, B. C.</dc:creator>
<dc:creator>Bessesen, D. H.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Boyd, G.</dc:creator>
<dc:creator>Buford, T. W.</dc:creator>
<dc:creator>Burant, C. F.</dc:creator>
<dc:creator>Carnero, E. A.</dc:creator>
<dc:creator>Carr, S.</dc:creator>
<dc:creator>Chambers, T. L.</dc:creator>
<dc:creator>Chavez, C.</dc:creator>
<dc:creator>Chen, H.</dc:creator>
<dc:creator>Chen, S.-H.</dc:creator>
<dc:creator>Christle, J. W.</dc:creator>
<dc:creator>Claiborne, A.</dc:creator>
<dc:creator>Clark, N</dc:creator>
<dc:date>2026-03-05</dc:date>
<dc:identifier>doi:10.64898/2026.03.02.705347</dc:identifier>
<dc:title><![CDATA[Molecular Transducers of Physical Activity Consortium (MoTrPAC): Initial Insights into the Dynamic Human Responses to Exercise]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2026-03-05</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2026.03.04.705181v1?rss=1">
<title>
<![CDATA[
Integrative Multi-omics Analysis of the Human Skeletal Muscle Response to Endurance or Resistance Exercise: Findings from the Molecular Transducers of Physical Activity Consortium (MoTrPAC) 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2026.03.04.705181v1?rss=1"
</link>
<description><![CDATA[
The Molecular Transducers of Physical Activity Consortium (MoTrPAC) was established to systematically characterize the molecular basis of the health benefits of exercise. Here, we present the integrative, multi-omics response of human skeletal muscle to acute endurance (EE) and resistance (RE) exercise. Distinct temporal responses were observed, with changes in ATAC-seq, phosphoproteome, and metabolome occurring before changes in the transcriptome and proteome. These distinct temporal multi-omic dynamics were used to identify transcriptional regulatory hubs converging around MEF2A and NFIC regulation of autophagy, angiogenesis and metabolism. Further, early RE-specific phosphoproteome signatures counteracted epigenetic modifications and downregulated transcripts involved in protein turnover. Additional findings include suppression of HIPK2/3 kinase signatures linked to the acute exercise regulation of sarcomeric proteins TTN, NEB, ANKRD2 and LMOD2. Our data demonstrate distinct temporal regulation across the multi-omic landscape of human skeletal muscle, with EE and RE eliciting common and unique molecular signatures.
]]></description>
<dc:creator>Keshishian, H.</dc:creator>
<dc:creator>Many, G. M.</dc:creator>
<dc:creator>Smith, G.</dc:creator>
<dc:creator>Clark, N. M.</dc:creator>
<dc:creator>Iyer, G.</dc:creator>
<dc:creator>Hart, P.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Montalvo, S.</dc:creator>
<dc:creator>Zhang, Z.</dc:creator>
<dc:creator>Jin, C.</dc:creator>
<dc:creator>Sanford, J. A.</dc:creator>
<dc:creator>Carr, S. A.</dc:creator>
<dc:creator>Adkins, J. N.</dc:creator>
<dc:creator>Mani, D. R.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Trappe, S.</dc:creator>
<dc:creator>Houmard, J. A.</dc:creator>
<dc:creator>Musi, N.</dc:creator>
<dc:creator>Huffman, K. M.</dc:creator>
<dc:creator>Kraus, W. E.</dc:creator>
<dc:creator>Sparks, L. M.</dc:creator>
<dc:creator>Thalacker-Mercer, A. E.</dc:creator>
<dc:creator>Sealfon, S. C.</dc:creator>
<dc:creator>Xia, A. Y.</dc:creator>
<dc:creator>Katz, D. H.</dc:creator>
<dc:creator>Newgard, C. B.</dc:creator>
<dc:creator>Burant, C. F.</dc:creator>
<dc:creator>Coen, P. M.</dc:creator>
<dc:creator>Goodpaster, B. H.</dc:creator>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:date>2026-03-06</dc:date>
<dc:identifier>doi:10.64898/2026.03.04.705181</dc:identifier>
<dc:title><![CDATA[Integrative Multi-omics Analysis of the Human Skeletal Muscle Response to Endurance or Resistance Exercise: Findings from the Molecular Transducers of Physical Activity Consortium (MoTrPAC)]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2026-03-06</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2026.03.02.704798v1?rss=1">
<title>
<![CDATA[
Blood Biochemical Responses to Acute Exercise: Findings from the Molecular Transducers of Physical Activity Consortium (MoTrPAC) 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2026.03.02.704798v1?rss=1"
</link>
<description><![CDATA[
Exercise benefits numerous organ systems and tissues, however limited knowledge exists about its underlying molecular pathways. Identifying the exercise-induced biochemical changes that occur in the circulation may provide further insights into how exercise confers systemic health changes. Here, we perform large-scale plasma proteomic, metabolomic, and whole blood transcriptional profiling in sedentary human participants undergoing acute endurance exercise (EE), resistance exercise (RE), or a non-exercise control (CON) in up to 7 timepoints over a 24 hour period. We observe 7066 transcript, 189 protein, and 448 metabolite changes in response to EE or RE compared to CON. Our analyses reveal numerous shared biochemical responses between EE and RE modes, but also differences in immune cell responses, lipid metabolism, and pathways reflective of tissue repair and angiogenesis. Taken together, our findings highlight novel temporal and exercise mode-specific blood-based molecular responses to acute exercise, and provide a new resource for the scientific community.
]]></description>
<dc:creator>Robbins, J. M.</dc:creator>
<dc:creator>Katz, D. H.</dc:creator>
<dc:creator>Many, G.</dc:creator>
<dc:creator>Rao, P.</dc:creator>
<dc:creator>Smith, G. R.</dc:creator>
<dc:creator>Tiwari, G.</dc:creator>
<dc:creator>Spielmann, G.</dc:creator>
<dc:creator>Montalvo, S.</dc:creator>
<dc:creator>Iyer, G.</dc:creator>
<dc:creator>Amar, D.</dc:creator>
<dc:creator>Leach, D. T.</dc:creator>
<dc:creator>Coyne, B. J.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Goodpaster, B. H.</dc:creator>
<dc:creator>Walsh, M. J.</dc:creator>
<dc:creator>Clish, C. B.</dc:creator>
<dc:creator>Burant, C. F.</dc:creator>
<dc:creator>Gerszten, R. E.</dc:creator>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:date>2026-03-04</dc:date>
<dc:identifier>doi:10.64898/2026.03.02.704798</dc:identifier>
<dc:title><![CDATA[Blood Biochemical Responses to Acute Exercise: Findings from the Molecular Transducers of Physical Activity Consortium (MoTrPAC)]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2026-03-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2026.02.27.702183v1?rss=1">
<title>
<![CDATA[
Multi-Omic, Multi-Tissue Responses to Acute Exercise in Sedentary Adults: Findings from the Molecular Transducers of Physical Activity Consortium 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2026.02.27.702183v1?rss=1"
</link>
<description><![CDATA[
Regular physical activity represents one of the greatest mechanisms for maintaining human health, yet the underlying molecular transducers of these benefits remain incompletely understood. Multi-omic assays now provide new opportunities to study the coordinated molecular responses of body tissues to different exercise modalities. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) was established to address this need by creating a molecular map of the response to physical activity. Described here is the first human cohort of MoTrPAC: sedentary adults enrolled prior to study suspension during the COVID-19 pandemic (N=175) randomized to either endurance or resistance exercise, or non-exercise control. From these participants, we detail their global acute molecular response in skeletal muscle, adipose tissue, and blood, integrated at multiple levels: tissue, exercise modality, timepoint, and omic category. These analyses characterize key molecular pathways, identify central regulators, and implicate novel candidate exerkines in mediating multi-organ exercise effects.
]]></description>
<dc:creator>MoTrPAC Study Group,</dc:creator>
<dc:creator>Katz, D. H.</dc:creator>
<dc:creator>Jin, C. A.</dc:creator>
<dc:creator>Many, G. M.</dc:creator>
<dc:creator>Smith, G. R.</dc:creator>
<dc:creator>Keshishian, H.</dc:creator>
<dc:creator>Clark, N. M.</dc:creator>
<dc:creator>Iyer, G.</dc:creator>
<dc:creator>Ahn, C.</dc:creator>
<dc:creator>Lindholm, M. E.</dc:creator>
<dc:creator>Sagendorf, T. J.</dc:creator>
<dc:creator>Amar, D.</dc:creator>
<dc:creator>Barber, J. L.</dc:creator>
<dc:creator>Brandt, A. R.</dc:creator>
<dc:creator>Coen, P. M.</dc:creator>
<dc:creator>Ge, Y.</dc:creator>
<dc:creator>Hart, P.</dc:creator>
<dc:creator>Hsu, F.-C.</dc:creator>
<dc:creator>Jaeger, B. C.</dc:creator>
<dc:creator>Jimenez-Morales, D.</dc:creator>
<dc:creator>Leach, D. T.</dc:creator>
<dc:creator>Mani, D. R.</dc:creator>
<dc:creator>Montalvo, S.</dc:creator>
<dc:creator>Pincas, H.</dc:creator>
<dc:creator>Rao, P.</dc:creator>
<dc:creator>Sanford, J. A.</dc:creator>
<dc:creator>Smith, K. S.</dc:creator>
<dc:creator>Vetr, N. G.</dc:creator>
<dc:creator>Adkins, J. N.</dc:creator>
<dc:creator>Ashley, E. A.</dc:creator>
<dc:creator>Carr, S. A.</dc:creator>
<dc:creator>Miller, M. E.</dc:creator>
<dc:creator>Montgomery, S. B.</dc:creator>
<dc:creator>Nair, V. D.</dc:creator>
<dc:creator>Robbins, J. M.</dc:creator>
<dc:creator>Snyder, M. P.</dc:creator>
<dc:creator>Sparks, L. M.</dc:creator>
<dc:creator>Tracy, R.</dc:creator>
<dc:creator>Walsh, M. J.</dc:creator>
<dc:creator>Wheeler, M. T.</dc:creator>
<dc:creator>Xia, A. Y.</dc:creator>
<dc:creator>Sealfon, S. C.</dc:creator>
<dc:creator>Gerszten, R</dc:creator>
<dc:date>2026-03-03</dc:date>
<dc:identifier>doi:10.64898/2026.02.27.702183</dc:identifier>
<dc:title><![CDATA[Multi-Omic, Multi-Tissue Responses to Acute Exercise in Sedentary Adults: Findings from the Molecular Transducers of Physical Activity Consortium]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2026-03-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://biorxiv.org/cgi/content/short/2026.03.05.702363v1?rss=1">
<title>
<![CDATA[
Multi-omic responses to acute exercise in abdominal subcutaneous adipose tissue of sedentary adults: findings from MoTrPAC 
]]>
</title>
<link>
https://biorxiv.org/cgi/content/short/2026.03.05.702363v1?rss=1"
</link>
<description><![CDATA[
Exercise induces widespread health benefits across multiple tissues, yet the acute molecular responses in human adipose tissue remain poorly defined. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) profiled temporal molecular changes in abdominal subcutaneous adipose tissue (ASAT) following a single bout of exercise. Healthy sedentary adults were randomized to endurance (EE), resistance (RE), or control (CON) groups. ASAT biopsies were collected pre-exercise and at 45min, 4hr, and 24hr post-exercise, followed by transcriptomic, proteomic, phosphoproteomic, and metabolomic analyses. EE and RE elicited distinct, time-resolved molecular programs involving angiogenesis, extracellular matrix remodeling, mitochondrial metabolism, substrate utilization, and circadian regulation. Phosphoproteomics revealed acute changes in cytoskeletal and branched-chain amino acid metabolism proteins associated with glycemic control. Temporal metabolomic shifts were cell-type-specific. Finally, we identified candidate adipose-derived exerkines with predicted endocrine actions. This multi-omic map of acute ASAT responses offers insight into adipose-specific mechanisms by which exercise promotes metabolic health.
]]></description>
<dc:creator>Ahn, C.</dc:creator>
<dc:creator>Jin, C. A.</dc:creator>
<dc:creator>Whytock, K. L.</dc:creator>
<dc:creator>Many, G. M.</dc:creator>
<dc:creator>Sagendorf, T. J.</dc:creator>
<dc:creator>Sanford, J. A.</dc:creator>
<dc:creator>Hou, Z.</dc:creator>
<dc:creator>Viggars, M. R.</dc:creator>
<dc:creator>Nie, J.</dc:creator>
<dc:creator>Espinoza, S.</dc:creator>
<dc:creator>Musi, N.</dc:creator>
<dc:creator>Sun, Y.</dc:creator>
<dc:creator>Pino, M. F.</dc:creator>
<dc:creator>Hart, P.</dc:creator>
<dc:creator>Katz, D. H.</dc:creator>
<dc:creator>Keshishian, H.</dc:creator>
<dc:creator>Smith, G. R.</dc:creator>
<dc:creator>Trappe, S.</dc:creator>
<dc:creator>Clark, N. M.</dc:creator>
<dc:creator>Bodine, S. C.</dc:creator>
<dc:creator>Goodyear, L. J.</dc:creator>
<dc:creator>Esser, K. A.</dc:creator>
<dc:creator>Newgard, C. B.</dc:creator>
<dc:creator>Bergman, B. C.</dc:creator>
<dc:creator>Adkins, J. N.</dc:creator>
<dc:creator>Walsh, M. J.</dc:creator>
<dc:creator>Sparks, L. M.</dc:creator>
<dc:creator>The MoTrPAC Group,</dc:creator>
<dc:date>2026-03-09</dc:date>
<dc:identifier>doi:10.64898/2026.03.05.702363</dc:identifier>
<dc:title><![CDATA[Multi-omic responses to acute exercise in abdominal subcutaneous adipose tissue of sedentary adults: findings from MoTrPAC]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory Press</dc:publisher>
<prism:publicationDate>2026-03-09</prism:publicationDate>
<prism:section></prism:section>
</item>
</rdf:RDF>
