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<title>bioRxiv Subject Collection: Genetics Plant Biology</title>
<link>https://biorxiv.org</link>
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This feed contains articles for bioRxiv Subject Collection "Genetics Plant Biology"
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<title>bioRxiv</title>
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<link>https://www.biorxiv.org</link>
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<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.02.748779v1?rss=1">
<title>
<![CDATA[
Wolf microevolution in the melting pot: range expansion, population sympatry, dynamic mosaic admixture zone and asymmetric gene flow 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.02.748779v1?rss=1
</link>
<description><![CDATA[
Here we describe substantial range shifts of historically differentiated wolf populations and formation of novel sympatric zones during the recent decade in Central Europe. This region provides a natural laboratory for testing alternative scenarios of population interactions from continued isolation or restricted gene flow to progressive population fusion, while prompting a reassessment of their geographic ranges. Based on sampling across the Czech Republic and Slovakia obtained from large-scale monitoring programmes over five wolf years (2020/21_2024/25), complemented by comparative material from neighbouring regions, we analysed mitochondrial haplotypes, autosomal microsatellite genotypes and sex-linked loci. The Central European population with Baltic ancestry predominated across large parts of Central Europe including the Bohemian Massif with enclaves in the Western Carpathians. The Carpathian population was predominant in Slovakia, with a smaller satellite occurrence in the northern part of the Bohemian Massif. Alpine population was centred in the Alps but extended into southern parts of Bohemian Massif and Central German Uplands. Following the sporadic occurrence of admixed individuals, broad mosaic and dynamic sympatric zones have formed in the Czech Republic and Slovakia in the last decade. These scenarios could be facilitated by the presence of intermediate habitats and isolation of the Bohemian Massif structural basin, framed by a massive ring fault system. Recent-immigration estimates are asymmetric, with the largest mean contributions from the Alpine to the Central European population and from the Central European to the Carpathian population, with the second case potentially linked to source-sink dynamics driven by the hunting pressure within the Carpathian population (whereas the others are protected year-round). Whether increasing admixture will enhance viability of populations (that currently have small effective sizes) through genetic rescue or carry risks of outbreeding depression remains uncertain, highlighting the need for continued transboundary monitoring within conservation biology framework.
]]></description>
<dc:creator><![CDATA[ Srutova, J., Tkacova, N., Cetkovska, E., Eliasova, K., Veselovska, L., Ungrova, L., Montoya, K., Skrobanek, M., Mateju, P., Dula, M., Vorel, A., Mokry, J., Mikslova, K., Collet, S., Nowak, C., Rolle, F., Marucco, F., Szewczyk, M., Myslajek, R., Nowak, S., Findo, S., Antal, V., Kutal, M., Jelinkova, J., Bolfikova, B. C., Hulva, P. ]]></dc:creator>
<dc:date>2026-09-04</dc:date>
<dc:identifier>doi:10.64898/2026.09.02.748779</dc:identifier>
<dc:title><![CDATA[Wolf microevolution in the melting pot: range expansion, population sympatry, dynamic mosaic admixture zone and asymmetric gene flow]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.02.748885v1?rss=1">
<title>
<![CDATA[
Evidence for a regulatory role of the evolutionarily conserved sequence complementarity between tRNAs 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.02.748885v1?rss=1
</link>
<description><![CDATA[
The tRNAs are non-coding RNAs (ncRNAs) known for their classical role in decoding mRNAs. Analysis of tRNA sequences from Escherichia coli revealed pairwise similarities for different combinations of tRNAs, as well as complementarities between some tRNA pairs. To explore the physiological significance of the complementarities between the tRNAs, we investigated the pair of tRNAs encoded by lysT (encoding an abundant tRNA, tRNALysT), and argU (encoding a rare tRNA, tRNAArgU). We show that tRNALysT and tRNAArgU anneal to form a heterodimer in vitro. The heterodimerisation is prevented by the presence of DNA oligomers complementary to the interacting sequences, in a dose dependent manner. Consistent with the notion of sequestration of tRNAArgU by tRNALysT, while the overexpression of tRNAArgU did not impact the culture growth, that of tRNALysT did. The tRNALysT mediated inhibition of the culture growth was enhanced at a lower temperature. The AGA minigene (decoded by tRNAArgU) mediated toxicity and hybrid phage ({lambda}imm-P22) growth on ssrA (tmRNA) strains was also consistent with the sequestration of tRNAArgU by tRNALysT. Also, we observed tRNA-derived fragments (tRFs) from tRNALysT and tRNAArgU, which too might facilitate tRNAArgU sequestration. Taken together, these observations support a novel regulatory role of the evolutionary conserved complementarities between tRNAs.
]]></description>
<dc:creator><![CDATA[ Sahu, A. K., Dash, A. A., Varshney, U. ]]></dc:creator>
<dc:date>2026-09-04</dc:date>
<dc:identifier>doi:10.64898/2026.09.02.748885</dc:identifier>
<dc:title><![CDATA[Evidence for a regulatory role of the evolutionarily conserved sequence complementarity between tRNAs]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.31.748413v1?rss=1">
<title>
<![CDATA[
EDTP Loss of Function Impairs Longevity and Reproduction 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.31.748413v1?rss=1
</link>
<description><![CDATA[
This study presents a comprehensive genetic characterization of DJ694, a viable GAL4 enhancer-trap allele of the age-regulated gene EDTP. EDTP transcript levels are reduced in DJ694, and homozygous flies exhibit reduced reproduction and shortened lifespan in both sexes. The fertility and longevity phenotypes are recessive and can be fully rescued by three independent UAS-EDTP insertions. Expression of UAS-EDTP into animals with wildtype phenotypes does not affect female fertility or lifespan. DJ694 has a jumpy behaviour but it is not detected by a locomotion assay. Like its human homolog, Muscle-specific Inositol Phosphatase (MIP, also known as MTMR14), EDTP is mainly expressed in adult muscles. The structure of the muscle, myofibril, and sarcomere appears normal in homozygous DJ694. DJ694 females have morphologically normal ovaries, implicating a functional rather than structural impairment. We demonstrate that EDTP is required during both development and adulthood to support normal fertility, with expression restricted to either stage alone being insufficient. Although it has been reported that EDTP can influence the accumulation of polyglutamine aggregates, we did not find evidence in its native tissue. Ectopic expression in the eye reduces the amount of aggregates but EDTP overexpression in muscles has no detectable effect on the accumulation or toxicity of two different kinds of polyglutamine aggregates.
]]></description>
<dc:creator><![CDATA[ Lu, X., Barwell, T., Edelman, S., Seroude, L. ]]></dc:creator>
<dc:date>2026-09-04</dc:date>
<dc:identifier>doi:10.64898/2026.08.31.748413</dc:identifier>
<dc:title><![CDATA[EDTP Loss of Function Impairs Longevity and Reproduction]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.31.748193v1?rss=1">
<title>
<![CDATA[
Implication of a rare variant in OPA1 in Cardiac Pathophysiology: From Cristae Remodelling to Contractile Dysfunction 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.31.748193v1?rss=1
</link>
<description><![CDATA[
Abstract Optic Atrophy 1 (OPA1), an important inner mitochondrial membrane GTPase, regulates mitochondrial fusion, maintains cristae structure, calcium buffering, cellular bioenergetics, preserves mtDNA and controls apoptosis. Here we examined the role of OPA1 variants in DCM using whole-exome sequencing (WES) of 5 familial and 10 sporadic DCM cases. A rare de novo OPA1 variant, c.563C>T (p.Pro188Leu), was identified in a DCM patient, which is absent in 100 healthy controls as well as in the 1000 Genomes, IndiGenomes and GenomeAsia 100k databases while it showed very low MAF (0.000069) in GnomAD. Structural modelling predicted the variant to be highly deleterious and revealed marked conformational distortion of the mutant protein (RMSD = 3.5 Angstrom). Molecular docking further demonstrated enhanced accessibility of mutant OPA1 to mitochondrial protease OMA1, suggesting increased OPA1 proteolytic processing and a consequent increase in mitochondrial fragmentation. Functional analysis in stable H9C2 cardiomyoblast cells, demonstrated significantly reduced OPA1 protein expression, extensive mitochondrial fragmentation in mutant-OPA1 expressing cells. The mutant protein caused significant reduction in mitochondrial membrane potential, ATP generation, and oxygen consumption rate (OCR), together with elevated cytosolic Calcium and reactive oxygen species (ROS) levels. qRT-PCR analysis further revealed depletion in mtDNA copy number and increased in expression of intrinsic apoptotic markers Caspase3, 9 and Bax/Bcl-2 ratio. The above findings collectively highlighted the significant impact of the OPA1 mutation on mitochondrial dynamics and cellular health, suggesting a significant correlation with the pathogenesis of DCM. Collectively, these findings suggest that OPA1-mediated mitochondrial dysfunction represents a potential therapeutic avenue for the management of DCM.
]]></description>
<dc:creator><![CDATA[ gupta, m., Mukhopadhyay, A., Kumar, A., Mohapatra, B. ]]></dc:creator>
<dc:date>2026-09-04</dc:date>
<dc:identifier>doi:10.64898/2026.08.31.748193</dc:identifier>
<dc:title><![CDATA[Implication of a rare variant in OPA1 in Cardiac Pathophysiology: From Cristae Remodelling to Contractile Dysfunction]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-04</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747754v1?rss=1">
<title>
<![CDATA[
The evolutionarily conserved EHMT1/G9a histone methyltransferase family regulates sleep maintenance through ROS homeostasis in insulin-producing cells 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747754v1?rss=1
</link>
<description><![CDATA[
Sleep disturbances are a common, still poorly characterized feature of Kleefstra syndrome (KLEFS1), a neurodevelopmental disorder caused by rare variants in the epigenetic regulator EHMT1. The gap in understanding the characteristics and origin of these sleep disturbances poses a major barrier for therapy development. In this cross-species study, we reveal that 70% of individuals with KLEFS1 experience severe sleep maintenance insomnia, marked by fragmented sleep due to frequent night awakenings. Furthermore, common genetic variation at the EHMT1 locus was associated with short sleep and insomnia symptoms in the general population. Drosophila mutants of the EHMT1 orthologue G9a recapitulate these phenotypes, exhibiting reduced and fragmented sleep. We show that G9a is required in insulin-producing cells (IPCs) and the fat body, in the latter during development, to ensure adult sleep integrity. Untargeted metabolomics revealed widespread metabolic dysregulation in G9a mutants, particularly affecting methionine metabolism. Mutants exhibited reduced methionine and elevated methionine sulfoxide (Met-SO), pointing to increased reactive oxygen species (ROS). Redox sensors revealed increased H2O2-dependent oxidation in the larval brain and an elevated glutathione redox potential in IPCs during development but not in adulthood. IPC-specific knockdown of MsrA, the enzyme that reduces Met-SO back to methionine, reproduced sleep fragmentation. Developmental, but not acute, antioxidant treatment fully restored adult sleep consolidation, demonstrating that G9a safeguards sleep via ROS homeostasis in early life. Finally, we show that a Drosophila sleep-restriction paradigm based on human sleep-restriction therapy can override the developmental defects and restore sleep continuity in adulthood. Our findings establish an evolutionarily conserved role for EHMT1/G9a in sleep regulation and provide a mechanistic framework to understand and treat sleep disturbances in KLEFS1.
]]></description>
<dc:creator><![CDATA[ Coll-Tane, M., van Renssen, L. V., Raun, N., Han, J., Ribas-Ros, N., van Reijmersdal, B., Luong, J., Pignato, C., Kampshoff, F., Gong, N. N., Jones, S. G., Pillen, S., Castells-Nobau, A., Mayneris-Perxachs, J., Klein, M., Kayser, M. S., Kleefstra, T., Schenck, A. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747754</dc:identifier>
<dc:title><![CDATA[The evolutionarily conserved EHMT1/G9a histone methyltransferase family regulates sleep maintenance through ROS homeostasis in insulin-producing cells]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1?rss=1">
<title>
<![CDATA[
Complementation of a Setaria Rubisco activase mutant with Agave Rubisco activase restores growth and photosynthesis 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747894v1?rss=1
</link>
<description><![CDATA[
Photosynthetic carbon assimilation is sensitive to heat stress, with a key component being the thermal sensitivity of Rubisco activase (RCA). One approach to increasing heat tolerance of photosynthesis is to increase the thermotolerance of RCA. Here, we have used a transgenic approach to express RCA{beta} from Agave tequilana (AtRCA) in Setaria viridis. A second line was created where the endogenous SvRCA{beta} was substituted for AtRCA{beta} through complementation of a null mutant, {triangleup}rcaB (AtRCA[bKO]). In vitro assays showed that Agave RCA readily activates Setaria Rubisco, and that its thermostability is higher than that of Setaria RCA. AtRCA[bKO] plants exhibited higher CO2 assimilation at 25{degrees}C compared to WT and AtRCA plants, although Rubisco content and activation did not change. After six hours of heat stress, AtRCA[bKO] plants retained higher CO2 assimilation rates than AtRCA, however, after 24 hours CO2 assimilation declined to similar levels in all lines, suggesting that RCA may not limit carbon assimilation at the conditions tested. Taken together, our results show that substitution of RCA from a CAM plant into a C4 model allows normal growth and supports slightly increased carbon assimilation under control and short-term heat conditions.
]]></description>
<dc:creator><![CDATA[ Hotto, A., Gartner, S., Eshenour, K., Stern, D. B. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747894</dc:identifier>
<dc:title><![CDATA[Complementation of a Setaria Rubisco activase mutant with Agave Rubisco activase restores growth and photosynthesis]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747924v1?rss=1">
<title>
<![CDATA[
ABF2 and bZIP2 remodel root system architecture under combined phosphate deficiency and salinity in Arabidopsis 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747924v1?rss=1
</link>
<description><![CDATA[
Climate change is degrading arable soils and imposing chronic abiotic stress on plants. As the primary interface with the soil, the root system is especially vulnerable, and plants respond by remodelling their root system architecture (RSA). Most studies have examined single stresses in isolation and so fail to capture the multifactorial environments in which plants actually grow. Rising salinity impairs development by compromising cellular integrity and triggering cytotoxic responses, and it simultaneously restricts uptake of phosphorus, a macronutrient required for nucleic acid and protein synthesis. Combined stresses can amplify these effects, yet how plants adjust RSA to cope with them remains poorly understood. Here we performed a meta-analysis of public transcriptomic datasets for salinity and phosphate deficiency in Arabidopsis thaliana. Integrating these data with protein-protein interaction modelling and DNA-binding (DAP-seq) analyses, we identified two basic leucine zipper transcription factors, ABF2 and bZIP2, as candidate integrators of the combined-stress response. Characterization of abf2, bzip2 and double mutants revealed RSA alterations and allowed us to identify downstream targets of each factor. Our findings offer new insight into root adaptation under combined stress and represent one of the few comprehensive analyses of how multiple abiotic stressors jointly shape root development and plant fitness.
]]></description>
<dc:creator><![CDATA[ Grenett, H. I., Johnson, N. R., Ibeas, M. A., Moyano, T. C., Perez-Diaz, J., Acha, R., Ahumada-Langer, L., Vasquez-Marambio, G., Kim, A.-R., Berdion Gabarain, V., Perrimon, N., Alvarez, J. M., Estevez, J. M. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747924</dc:identifier>
<dc:title><![CDATA[ABF2 and bZIP2 remodel root system architecture under combined phosphate deficiency and salinity in Arabidopsis]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747630v1?rss=1">
<title>
<![CDATA[
Nutrient Concentration Declines but Nutrient Yield Increases Under Elevated CO2 Concentration in Soybean: Disentangling the relative effects of Dilution, Transpiration, and Uptake Activity 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747630v1?rss=1
</link>
<description><![CDATA[
Rising atmospheric CO2 concentrations consistently reduce mineral concentrations in C crops, yet the mechanisms driving this decline - dilution, reduced transpiration-driven mass flow, and altered root nutrient acquisition capacity - have rarely been tested simultaneously under field conditions. Here, we grew two soybean (Glycine max Merr.) varieties with contrasting yield responses to elevated [CO2] (Loda and HS93-4118) at the Soybean Free Air CO2 Enrichment (SoyFACE) facility and quantified the independent contributions of dilution, transpiration, and root uptake to observed changes in tissue mineral concentration and nutrient yield. Elevated [CO2] increased grain yield by 21% and 6% in Loda and HS, respectively, and reduced transpiration by 13-14% across both varieties. A decomposition analysis revealed that while dilution and reduced transpiration each exerted negative effects on tissue nutrient concentrations, with their combined effect representing a 31-40% potential reduction in whole-plant concentration, active root uptake responses were positive for nearly all nutrients, largely offsetting these losses and resulting in observed concentration declines substantially smaller than either mechanism alone would predict. Consequently, whole-plant nutrient yield increased under elevated CO2 for all elements measured, and grain nutrient yield increased for most. The relationship between seasonal transpiration and nutrient yield was steeper under elevated CO2 for most macronutrients, indicating that plants acquired more nutrients per unit water transpired, not less, under elevated CO2. These results demonstrate that root uptake is the dominant compensatory response to elevated [CO2] driven dilution and reduced mass flow in soybean, that nutrient yield is maintained or increased for most elements under elevated [CO2], and that Fe represents a physiologically and nutritionally significant vulnerability in future CO2 environments.
]]></description>
<dc:creator><![CDATA[ Kwafo, T. S. K., Yerkes, K., McGrath, J. M. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747630</dc:identifier>
<dc:title><![CDATA[Nutrient Concentration Declines but Nutrient Yield Increases Under Elevated CO2 Concentration in Soybean: Disentangling the relative effects of Dilution, Transpiration, and Uptake Activity]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.31.748199v1?rss=1">
<title>
<![CDATA[
Petal photosynthesis and dynamic alterations of carbon metabolism orchestrate floral maturation in Gardenia carinata 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.31.748199v1?rss=1
</link>
<description><![CDATA[
During maturation, flowers exhibit complex and finely-coordinated metabolic events facilitating various physiological changes that occur in a short time-span. The floral carbon metabolism underpins a metabolic frame, which provides energy and metabolites to drive the life-processes in flowers. However, studies on floral maturation in the past mostly focused on specialized metabolism or certain metabolite classes of central carbon metabolism. This study aims to provide a detailed insight into the dynamics of floral carbon metabolism and its role in flower maturation; it also addresses a long-standing question on the role of petal photosynthesis in floral maturation. In this report, time-course metabolomic studies were integrated with various physiological, enzymological, histochemical and ultrastructural findings to present a multi-layered investigation on the maturation physiology of Gardenia carinata flowers. This integrated approach revealed the characteristic metabolic features of different floral maturation stages, and also highlighted the metabolic rearrangements that allow each stage to establish their characteristic metabolic state. The photosynthetic bud stages showed metabolism inclined toward active growth and carbon reserve accumulation. A shift to energy-rich metabolism was observed as the flowers unfurled, probably to support energy-demanding events such as flower opening and changes in specialized metabolism. Our study further demonstrates the mechanisms underlining the transition from autotrophic to heterotrophic metabolism during maturation, which also reflects the plasticity in source-sink dynamics of flowers. We envisage that this study could provide a springboard for elucidating the roles of individual metabolites and enzymes in regulating floral maturation.
]]></description>
<dc:creator><![CDATA[ Ghosh, R., Mitra, A. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.31.748199</dc:identifier>
<dc:title><![CDATA[Petal photosynthesis and dynamic alterations of carbon metabolism orchestrate floral maturation in Gardenia carinata]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.02.748994v1?rss=1">
<title>
<![CDATA[
DNA Damage and Repair Mechanisms in Duckweed (Spirodela polyrhiza) Under Ultraviolet-B (UV-B) Light Stress 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.02.748994v1?rss=1
</link>
<description><![CDATA[
Exposure to Ultraviolet-B (UV-B) light can adversely affect plant growth and cellular integrity by inducing oxidative stress and DNA damage. In this study, we investigated UV-B-induced DNA damage and repair responses in the aquatic monocotyledonous plant species Spirodela polyrhiza (duckweed). We exposed 13-day-old duckweed plantlets to broadband UV-B light for 1-10 min, followed by recovery periods of up to 24 h under normal growth conditions. We observed progressive chlorosis, wilting, and diminished plant vigor with longer durations of UV-B light exposure. Agarose gel electrophoresis demonstrated compromised genomic DNA integrity immediately after UV-B light treatment, with partial restoration of DNA quality during recovery. Immuno-slot blot assays established the accumulation of two major UV light-induced photoproducts, cyclobutane pyrimidine dimers (CPDs) and 6-4 pyrimidine-pyrimidone photoproducts [(6-4)PPs], in a dose-dependent manner following UV-B light exposure. Notably, the abundance of these DNA lesions declined substantially after recovery, indicating activation of endogenous DNA repair mechanisms. Staining with 3,3-diaminobenzidine revealed elevated accumulation of hydrogen peroxide immediately following UV-B exposure, suggesting enhanced oxidative stress. Collectively, these findings demonstrate that S. polyrhiza possesses efficient mechanisms for sensing, repairing, and mitigating DNA damage induced by oxidative stress resulting from UV-B light exposure. This study highlights the potential of duckweed as an effective model system for investigating DNA damage and repair pathways under UV-B light stress in plants.
]]></description>
<dc:creator><![CDATA[ Sanjaya, M. A., Patil, S. S. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.02.748994</dc:identifier>
<dc:title><![CDATA[DNA Damage and Repair Mechanisms in Duckweed (Spirodela polyrhiza) Under Ultraviolet-B (UV-B) Light Stress]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.31.748265v1?rss=1">
<title>
<![CDATA[
Optimizing DNA extraction from environmentally degraded bone samples for molecular identification of cetacean species 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.31.748265v1?rss=1
</link>
<description><![CDATA[
Molecular identification of cetacean bone remains can be limited by DNA degradation and the presence of PCR inhibitors. Here, we present an optimized DNA extraction protocol based on a total demineralization method for environmentally exposed cetacean bones. The protocol uses 100 mg of bone powder, 24 h digestion with EDTA, N-lauroylsarcosine, and proteinase K, followed by a modified silica-column purification. Nine environmentally degraded bone samples representing eight individuals were processed. DNA concentrations ranged from 7.3 to 57.1 ng/uL (mean SD = 25.91- 13.91 ng/uL). The mitochondrial cytochrome b gene was successfully amplified from all samples using conventional PCR, and five samples (55.6%) yielded sequences suitable for downstream analysis. BLASTn identified Balaenoptera physalus as the closest database match for all recovered sequences, and phylogenetic analysis further supported their association with B. physalus reference sequences. These results demonstrate that the proposed protocol provides a practical approach for recovering amplifiable and molecularly informative mitochondrial DNA from environmentally degraded cetacean bone material, facilitating molecular identification from challenging skeletal remains.
]]></description>
<dc:creator><![CDATA[ Gamboa, M., Pagani, D., Rodriguez, S. M. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.31.748265</dc:identifier>
<dc:title><![CDATA[Optimizing DNA extraction from environmentally degraded bone samples for molecular identification of cetacean species]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.02.748781v1?rss=1">
<title>
<![CDATA[
TALE-independent transcriptional activation of the rice executor gene Xa23 is regulated via histone acetylation during zygote development 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.02.748781v1?rss=1
</link>
<description><![CDATA[
Transcription activator-like effectors (TALEs) from Xanthomonas activate transcription of executor (E) genes in host plants, leading to cell death and thereby restricting proliferation of biotrophic pathogens. Because E gene transcripts had only been detected upon activation by cognate Xanthomonas TALEs, E genes were thought to function exclusively in plant immunity. Here, we detect TALE-independent transcription of the rice E gene Xa23 in zygotes 4-6 hours after gamete fusion. Histone deacetylase inhibition induces Xa23 transcription in unfertilized egg cells, implicating histone acetylation in Xa23 regulation. We identified potential cis-regulatory elements and transcription start sites associated with native Xa23 transcription during zygote development. Together, our findings suggest that Xa23 is a developmentally regulated gene with a native role during early zygote development. This supports a previously proposed model in which E genes have native functions in development, while fortuitous upstream polymorphisms can create TALE-binding sites that convert them into immune executors.
]]></description>
<dc:creator><![CDATA[ Schenstnyi, K., Rattanawong, K., Satoh, A., Strauss, A., Faiss, N., Holmes, D. R., Redzich, L., Toda, E., Aini, H., Kinoshita, A., Lahaye, T., Okamoto, T. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.02.748781</dc:identifier>
<dc:title><![CDATA[TALE-independent transcriptional activation of the rice executor gene Xa23 is regulated via histone acetylation during zygote development]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.02.748918v1?rss=1">
<title>
<![CDATA[
Stomatal and xylem plasticity, not growth rate, determines white spruce resilience to warmer and drier climates 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.02.748918v1?rss=1
</link>
<description><![CDATA[
In a warmer and drier climate, forest productivity will depend on trees' ability to maintain carbon uptake and hydraulic function. Whether fast-growing genotypes of boreal conifers are more vulnerable to combined climatic stress remains uncertain. Using a full-factorial field experiment, we investigated how progressive soil drying combined with extended warming affects growth, xylem development, and photosynthesis in two Picea glauca families with contrasting growth strategies. Rainout structures first reduced soil moisture from 25% to 18%, followed by a +5{degrees}C warming treatment applied using infrared heaters. During the warmest and driest period in August, air temperature reached 34.5{degrees}C in the warmed plots, while soil moisture declined to a low of 15% in the combined rainout and warming treatment. Contrary to expectations, both fast- and slow-growing white spruce families exhibited similar resilience to concurrent warming and soil drying. This finding challenges the prevailing theory that faster growth increases vulnerability to climatic stress. Despite an approximately 50% reduction in rainfall, pre-dawn water potential remained above -0.5 MPa across treatments, reflecting that seedlings were able to avoid hydraulic stress. Although the fast-growing family maintained greater height and diameter growth compared to the slow-growing family, both exhibited similar physiological and anatomical responses to warming. Warming decreased stomatal conductance, which increased intrinsic water-use efficiency. Latewood xylem traits related to hydraulic efficiency were also reduced under warming. Together, these coordinated stomatal and xylem adjustments decreased water loss and protected hydraulic function, enabling both families to maintain high photosynthesis and growth under simulated climate conditions. Overall, white spruce exhibits strong phenotypic plasticity, supporting intraspecific resilience to moderate warming and soil drying representative of projected 21st-century summer conditions for central and eastern Canada.
]]></description>
<dc:creator><![CDATA[ Murphy, B. K., Perkins, N., Nagi, F., Wang, S., Muchos, T., Boyle, J. A., Isabel, N., Ensminger, I. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.02.748918</dc:identifier>
<dc:title><![CDATA[Stomatal and xylem plasticity, not growth rate, determines white spruce resilience to warmer and drier climates]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748396v1?rss=1">
<title>
<![CDATA[
Timing of transient darkness shapes carbon-nitrogen metabolism and sugar signaling in sugarcane 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748396v1?rss=1
</link>
<description><![CDATA[
Fluctuating light is common in field environments. Yet, the mechanisms by which C4 crops coordinate carbon and nitrogen metabolism during short-term carbon deprivation remain poorly understood. Here, we imposed transient darkness at different phases of the diel cycle to assess how the timing of light loss affects photosynthesis, carbohydrate turnover, amino acid dynamics, and sugar-sensing pathways in commercial sugarcane leaves. Early-day darkness significantly impaired photosynthetic induction and revealed a temporal disconnect between stomatal and metabolic limitations, whereas midday and late-day treatments caused temporary, time-specific disruptions in carbon assimilation. These shifts altered the balance between sucrose preservation and catabolic mobilization, leading to treatment-dependent changes in starch reserves and free amino acids. Core circadian components largely maintained their phase relationships, but their amplitudes varied across treatments, consistent with partial decoupling from carbon status. Darkness also reorganized energy signaling, with SnRK1 and DIN6 responses associated with greater declines in sucrose. Notably, trehalose-pathway transcripts showed marked changes in network connectivity, with ScTPSIIG consistently emerging as a highly connected candidate associated with photosynthetic performance, water-use traits, sugar sensing, and amino acid metabolism. Overall, these results indicate that the timing of carbon limitation and residual sucrose availability shape distinct metabolic responses, while trehalose metabolism provides a candidate regulatory layer coordinating carbon-nitrogen adjustment during the diel cycle, highlighting class II TPS proteins as targets for functional investigation of metabolic resilience in sugarcane.
]]></description>
<dc:creator><![CDATA[ Leal, G. M., de Oliveira, H. O., Farineli, F., Vanni-Lopes, A., Mira, W. V. M., Macedo, A. F., Romim, G. H., Hotta, C. T., Floh, E. I. S., Navarro, B. V., Buckeridge, M. S. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748396</dc:identifier>
<dc:title><![CDATA[Timing of transient darkness shapes carbon-nitrogen metabolism and sugar signaling in sugarcane]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747747v1?rss=1">
<title>
<![CDATA[
Genetic diversity within and between polyploid sugarcane (Saccharum spp.) families obtained via caryopsis using microsatellite markers and multicategory model 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747747v1?rss=1
</link>
<description><![CDATA[
Genetic diversity analyses are essential for sugarcane (Saccharum spp.) breeding programs. Crossbreeding, based on genetic distances between parental plants, is a tool used to increase genetic variability and enhance plant selection; however, quantifying variation in highly polyploid species remains a challenge. The present study aimed to evaluate the diversity within and between 12 families of sugarcane derived from caryopses, analyzing 120 individual seedlings arranged in an augmented block design. Genotyping was performed using primers for 16 microsatellite loci, five simple sequence repeat (SSR) loci, and 11 expressed sequence tag-SSR (EST-SSR) loci. To accurately account for polyploidy, similarity calculations were performed using Bruvos distances among individuals and RST distances among the families. Analysis of molecular variance (AMOVA) indicated that most of the genetic variability was within families (72%), with only 28% found between them. This high level of intra-family variation demonstrates that a significant reservoir of genetic diversity remains available within the crosses. The highest genetic similarity was observed between the families RB986952 x RB986960 and RB036122 x RB03611, whereas the lowest genetic similarity was observed between the families RB97319 x RB966928 and RB106802 x RB855036. Although the evaluated families shared high genetic similarity, the pronounced genetic variation within them demonstrates a robust recombination potential, indicating that the genetic basis of sugarcane can be better explored using the high variability that already exists in the selection of desirable morpho-agronomic characteristics within the families. Furthermore, this study highlights the importance of using appropriate distances for diversity studies with codominant markers, such as microsatellites, in polyploid species.
]]></description>
<dc:creator><![CDATA[ da Mata Borsuk, L. G., Zeni Neto, H., BIALETZKI CRISTIANO, V., Pires da Silva Machado, M. d. F., Aparecida Mangolin, C., Martins Montini, L., Cristina da Silva, J., Frederico dos Santos, R. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747747</dc:identifier>
<dc:title><![CDATA[Genetic diversity within and between polyploid sugarcane (Saccharum spp.) families obtained via caryopsis using microsatellite markers and multicategory model]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1?rss=1">
<title>
<![CDATA[
VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1?rss=1
</link>
<description><![CDATA[
Plants have remarkable capacity to reconstruct entire organ systems from tissue explants. In Arabidopsis two-step tissue culture system, pluripotency regulators are specifically expressed in the middle-cell layer of the stratified callus tissue. However, regulatory mechanisms underlying the radial patterning of callus remained unclear. Here, we found that very-long-chain fatty acids (VLCFAs) synthesized in the epidermis-like outermost layer are essential for pluripotency acquisition and successful shoot regeneration. Our genetic and transcriptomic analyses revealed that the regulatory roles of VLCFAs on pluripotency acquisition involve inter-cell layer signaling in callus tissue, while they are at least partly independent of ATML1/PDF2 functions and cuticular wax synthesis in the outermost layer. VLCFAs spatially restrict procambium cell identity by non-cell-autonomously suppressing cytokinin signaling, thereby allowing for establishment of the middle-cell layer. We propose that the inhibitory relationships between layer-specific regulators underlie the intricate balance of cellular fate determination in pluripotent callus.
]]></description>
<dc:creator><![CDATA[ Doll, Y., Nobusawa, T., Kojima, M., Nagata, K., Matsuda-Ito, K., Mähönen, A. P., Matsuda, T., Abe, M., Sakakibara, H., Ikeuchi, M. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748728</dc:identifier>
<dc:title><![CDATA[VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748354v1?rss=1">
<title>
<![CDATA[
Implementation and calibration of the Vaganov-Shashkin model in the virtualRings R package 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748354v1?rss=1
</link>
<description><![CDATA[
Process-based tree growth models provide a mechanistic framework for investigating how climate conditions regulate tree growth across daily to annual time scales. Yet, their broader application across species and environments is constrained by the limited accessibility in open-source environments and the difficulty of estimating physiological parameters that are rarely measured directly. Here, we present virtualRings, a new R package integrating the Vaganov-Shashkin model (VSM) and the RINGS3 models, and focus on the implementation and calibration of VSM. Using tree-ring width observations from seven Northern Hemisphere sites across various environmental conditions, we compared the traditional bootstrap-based calibration approach with the Covariance Matrix Adaptation Evolution Strategy (CMA-ES). CMA-ES improved agreement between simulated and observed radial tree growth and provided an efficient approach for model parameter estimation. We further evaluated practical CMA-ES settings to balance computational cost and performance and discussed its potential limitations. The virtualRings package provides an open and reproducible platform for tree growth simulation, facilitating the application of important process-based models across species and environments and the investigation of how temperature and moisture constraints regulate daily tree-ring formation across spatial and temporal scales.
]]></description>
<dc:creator><![CDATA[ Wang, F., Atkins, J. W., Anchukaitis, K. J., Wise, E. K., Jiang, X., Yang, B., Arseneault, D., Boucher, E., Dannenberg, M. P. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748354</dc:identifier>
<dc:title><![CDATA[Implementation and calibration of the Vaganov-Shashkin model in the virtualRings R package]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748733v1?rss=1">
<title>
<![CDATA[
OsPATROL1 overexpression accelerates stomatal opening to enhance photosynthetic induction and growth under fluctuating light in rice 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748733v1?rss=1
</link>
<description><![CDATA[
Slow stomatal opening after increases in irradiance constrains carbon gain under fluctuating light, yet stomatal kinetics remain an underexplored target for crop improvement. Here, we investigated Oryza sativa PROTON ATPASE TRANSLOCATION CONTROL 1 (OsPATROL1), which encodes a Munc13-like protein implicated in stomatal regulation in Arabidopsis thaliana. OsPATROL1 overexpression had modest, condition-dependent effects on steady-state gas exchange and did not alter stomatal morphology or biochemical traits. In contrast, it consistently accelerated stomatal opening and photosynthetic induction, reducing the stomatal conductance time constant during induction by 41-43%. During 12 h of simulated natural fluctuating light, OsPATROL1-overexpressing plants maintained higher stomatal conductance and net CO2 assimilation rate, increasing cumulative assimilation by 8-12% while maintaining their intrinsic water-use efficiency (iWUE). Under artificial fluctuating light, overexpression alleviated growth reductions relative to steady light. Under glasshouse conditions, total biomass increased by 34-44%, accompanied by greater tiller number, root biomass, bleeding sap rate, and leaf nitrogen content. Taken together, these results indicate that OsPATROL1 overexpression accelerates stomatal opening, enhances photosynthetic induction and daytime carbon gain without compromising iWUE, and is associated with greater growth.
]]></description>
<dc:creator><![CDATA[ Katsuhama, N., Morita, R., Wakabayashi, Y., Park, S., Fukayama, H., Aoki, N., Terashima, I., Yamori, W. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748733</dc:identifier>
<dc:title><![CDATA[OsPATROL1 overexpression accelerates stomatal opening to enhance photosynthetic induction and growth under fluctuating light in rice]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747797v1?rss=1">
<title>
<![CDATA[
In-cell structural analysis reveals a distinctive chloroplast ribosome in Chlamydomonas reinhardtii 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747797v1?rss=1
</link>
<description><![CDATA[
Chloroplast ribosomes synthesize plastid-encoded components of photosynthetic machinery, yet their structure and organization remain poorly understood. We combined cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine native chloroplast ribosomes in Chlamydomonas reinhardtii. The 4.4-4.9 [A] structure revealed a large arch-like extension on the small subunit (SSU). Comparisons with bacterial and plant chloroplast ribosomes, supported by proteomics, AlphaFold3 predictions and a recent atomic model, indicate that the arch is formed by insertions and extensions in SSU proteins. Classification resolved active, thylakoid-associated ribosomes with density adjacent to the nascent peptide exit and an arch-moved state enriched among thylakoid-associated particles, with coordinated displacement of the arch and beak. Phylogenetic analysis revealed an evolutionary mosaic: the uS3c insertion is broadly distributed across Chlorophyceae, whereas the uS2c insertion, uS5c and PSRP7 are concentrated in Chlamydomonadales, with PSRP7 also in Sphaeropleales. Nuclear-encoded components were recruited stepwise onto a plastid-encoded scaffold, with all four under comparable purifying selection. These findings link a lineage-specific SSU extension to ribosome dynamics, thylakoid association and evolution, highlighting the value of in-cell structural analysis.
]]></description>
<dc:creator><![CDATA[ Zhang, P., Hou, Z., Shen, Y., Zhang, Z., Lu, P., Katzourakis, A. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747797</dc:identifier>
<dc:title><![CDATA[In-cell structural analysis reveals a distinctive chloroplast ribosome in Chlamydomonas reinhardtii]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748463v1?rss=1">
<title>
<![CDATA[
A CO2-limitation-induced cytosolic repressor enables shutdown of the algal CO2-concentrating mechanism 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748463v1?rss=1
</link>
<description><![CDATA[
Aquatic photosynthetic organisms face limited CO2 availability because CO2 diffuses slowly in water and most dissolved inorganic carbon (Ci) exists as HCO3- at physiological pH. To overcome this limitation, aquatic photoautotrophs operate CO2-concentrating mechanisms (CCMs) that elevate CO2 around Rubisco and sustain carbon fixation. Because CCM operation consumes energy, it must be suppressed when CO2 becomes abundant, but how this shutdown occurs remains poorly understood. In Chlamydomonas reinhardtii, the nuclear protein CBP1 was identified as a CCM repressor, but its loss causes only partial derepression under high CO2, indicating that an additional mechanism is required for complete shutdown. Here, we identify High-Affinity CCM Repressor 1 (HCR1), a cytosolic protein related to CBP1, as a second repressor. Under high CO2, hcr1 mutants retained high affinity for Ci and derepressed CCM and photoacclimation genes. Combined disruption of HCR1 and CBP1 further increased Ci affinity, approaching that of wild-type cells with a fully induced CCM under CO2 limitation, and promoted the accumulation of Ci transporters. HCR1 loss also prevented redistribution of the chloroplast regulator CAS away from the pyrenoid and was accompanied by retention of a pyrenoid starch sheath. In contrast, LCIB, a chloroplast CO2-recapture protein, relocated normally. Unexpectedly, HCR1 accumulated during CO2 limitation and declined after transfer to high CO2. These results show that CCM shutdown is an active transition rather than the passive reversal of induction. We propose that CBP1 restrains CCM1-dependent transcription, while HCR1 is preloaded during CO2 limitation to terminate the CAS-associated, starch-sheathed, high-affinity state when CO2 becomes replete.
]]></description>
<dc:creator><![CDATA[ Miichi, S., Shimamura, D., Yasuda, J., Tokutsu, R., Yamano, T. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748463</dc:identifier>
<dc:title><![CDATA[A CO2-limitation-induced cytosolic repressor enables shutdown of the algal CO2-concentrating mechanism]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1">
<title>
<![CDATA[
The histone demethylase Kdm5 and the ARGONAUTE proteins Piwi and Aubergine regulate female abdominal pigmentation in Drosophila melanogaster 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.27.747613v1?rss=1
</link>
<description><![CDATA[
Insect pigmentation is an ecologically critical trait influencing many physiological processes. In Drosophila melanogaster, abdominal pigmentation is sexually dimorphic: males have fully pigmented posterior segments, while females exhibit a posterior melanin stripe. Pigmentation relies on the expression of pigmentation genes that encode enzymes involved in pigment synthesis. These genes are tightly regulated during pupal and young adult stages. To expand the gene regulatory network of pigmentation genes, we conducted an RNAi screen using the yellow-Gal4 driver, expressed during the pupal stage in abdominal epidermis. One of the candidates from this screen, Kdm5, encodes a histone demethylase erasing the H3K4me3 histone mark catalyzed by the histone methyl-transferase Trithorax (Trx). We show that Kdm5 down-regulation reduces abdominal pigmentation, mimicking trx down-regulation. Kdm5 activates melanin production through regulation of the pigmentation gene tan. Transcriptomic analyses reveal that Kdm5 and Trx share many targets in pupal abdominal epidermis, including piRNA pathway components such as piwi and aubergine. These piRNA components, originally associated with transposon silencing in the germline, also function in some somatic tissues such as the nervous system, the fat body or the gut. We demonstrate that Piwi and Aubergine participate in female abdominal pigmentation establishment, without evident piRNA production. We also show that Kdm5 and Piwi act not only in pupal abdominal epidermis but also in pupal fat body. This study therefore expands the regulatory network of pigmentation genes. It identifies a new somatic function for Kdm5 and Piwi and reveals a role for pupal fat body in female abdominal pigmentation regulation.
]]></description>
<dc:creator><![CDATA[ Narbey, R., De castro, S., Louvet-Vallee, S., Gho, M., Peronnet, F., Gibert, J.-M., Mouchel-Vielh, E. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.08.27.747613</dc:identifier>
<dc:title><![CDATA[The histone demethylase Kdm5 and the ARGONAUTE proteins Piwi and Aubergine regulate female abdominal pigmentation in Drosophila melanogaster]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748544v1?rss=1">
<title>
<![CDATA[
Salicylic acid-triggered apoplastic proteolysis releases cryptic phytocytokines with distinct immunogenic functions 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748544v1?rss=1
</link>
<description><![CDATA[
Plants rely on an innate immune system to defend against pathogens through various molecular responses. In addition to classical damage- and pathogen-associated molecular patterns (DAMPs and PAMPs), plants produce endogenous signaling peptides termed phytocytokines that amplify and regulate immune responses following stress. Although most characterized phytocytokines originate from dedicated precursor proteins, the contribution of multifunctional proteins to phytocytokine generation remains poorly understood. Here, we show that salicylic acid (SA) rapidly remodels the maize apoplastic peptidome through an early, transient proteolytic program driven by apoplastic serine hydrolases. Time course peptidomics identified fourteen candidate phytocytokines, including two cryptic peptides, PC13 and PC14, released from the stress-associated zinc-finger protein ZmSAP7 and the migration inhibitory factor-like protein ZmMDL1, respectively. Both peptides activated immune-associated gene expression but triggered distinct transcriptional responses and exerted opposing effects on Ustilago maydis infection, with PC13 enhancing resistance and PC14 promoting susceptibility. Biochemical analysis demonstrated that PMSF-sensitive apoplastic serine proteases directly process ZmMDL1 to release PC14. Together, our findings uncover a SA-responsive proteolytic pathway that generates functionally distinct phytocytokines from multifunctional proteins, expanding the repertoire of immune signaling peptides and revealing an additional layer of regulation in plant defense.
]]></description>
<dc:creator><![CDATA[ Katzy, P., Laia, H., Zick, Z., Misas Villamil, J. C., Doehlemann, G. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748544</dc:identifier>
<dc:title><![CDATA[Salicylic acid-triggered apoplastic proteolysis releases cryptic phytocytokines with distinct immunogenic functions]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748704v1?rss=1">
<title>
<![CDATA[
Arabidopsis thaliana ACTIN DEPOLYMERIZING FACTORs are novel susceptibility factors for Colletotrichum higginsianum 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748704v1?rss=1
</link>
<description><![CDATA[
Colletotrichum higginsianum (Ch) is a hemibiotrophic fungal pathogen that infects Brassicaceae plants, including Arabidopsis thaliana. The molecular mechanisms underlying the Ch-A. thaliana interaction are not fully understood. Particularly, the susceptibility factor against Ch infection remains to be determined. Here, we report that A. thaliana ACTIN DEPOLYMERIZING FACTORs (ADFs), ancient proteins that regulate the organization and dynamics of actin filaments (AFs), function as susceptibility factors during Ch infection. Among 11 ADFs encoded in A. thaliana genome, subclass I ADFs that include ADF1, -2, -3, and -4, express throughout the plant. We found that knockout mutant of ADF4 and transgenic plants in which the expression of all of subclass I members is suppressed (ADF1-4Ri) exhibited increased resistance to Ch. Cytological analyses revealed that both Ch penetration and secondary hyphae formation were suppressed in adf4 and ADF1-4Ri. This enhanced resistance was associated with suppression of Ch-induced AF fragmentation. In addition, we found that PENETRATION 2 (PEN2) plays a critical role in the Ch resistance in adf4 and ADF1-4Ri. Our findings suggest that subclass I ADFs promote AF fragmentation during Ch infection, thereby suppressing PEN2-associated mitochondria accumulation at Ch entry sites. Together, these results raise the possibility that Ch exploits host ADF-dependent actin regulation to facilitate successful infection.
]]></description>
<dc:creator><![CDATA[ Ohashi, M., Aoki, S., Shimada, T. L., Ueda, T., Umeda, M., Inada, N. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748704</dc:identifier>
<dc:title><![CDATA[Arabidopsis thaliana ACTIN DEPOLYMERIZING FACTORs are novel susceptibility factors for Colletotrichum higginsianum]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748533v1?rss=1">
<title>
<![CDATA[
Loss of ELM1B impairs mitochondrial fission, matrix redox state and stress tolerance in Physcomitrium patens 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748533v1?rss=1
</link>
<description><![CDATA[
Mitochondria are endosymbiont-derived organelles that play a central role in cellular metabolism, energy production and stress responses. While single mitochondria represent functional units, they continuously exchange their contents through fusion and fission, facing stress conditions as a dynamic population. To date, it remains largely unknown how stress alters mitochondrial dynamics in plants and how altered dynamics affect mitochondrial properties and plant stress resilience. Here, we investigate mitochondrial dynamics in response to oxidative stress in the non vascular model plant Physcomitrium patens. By creating mutants with impaired mitochondrial fission in different reporter lines for mitochondrial parameters, we additionally analyse effects of chronic changes to mitochondrial population dynamics. We found that Mito-Paraquat (MtPQ) treatment increased the glutathione redox potential EGSH in mitochondria, the cytosol and chloroplasts, as monitored via roGFP2-based genetically encoded biosensors. Mitochondria elongated within hours and showed a concomitant and heterogenous increase of matrix EOSred, that we propose as a marker for matrix protein damage. Mitochondrial fission mutants lacking PpELM1B (ELONGATED MITOCHONDRIA) displayed distinct changes of mitochondrial morphology parameters as determined by automated 3D-segmentation and feature mapping (MorphoMapper) of confocal z-stacks. Elongated mitochondria in Ppelm1bge lines showed an oxidative matrix EGSH shift and increased matrix EOSred while matrix mixing still occurred, albeit at the same slow rate as in wildtype, within days. Macroscopically, Ppelm1bge lines displayed reduced growth, decreased respiration, and a higher sensitivity to oxidative stress. Our results show that plant mitochondrial morphology and physiological parameters specifically shift in response to stress and impaired fission. Mitochondrial fission is vital to maintain a healthy mitochondrial population that sustains plant oxidative stress tolerance.
]]></description>
<dc:creator><![CDATA[ Tamanna, S. S., Pompejus, S., Thangamani, S., Gadoud, C., Nermerich, I., Mühlhaus, T., Müller-Schüssele, S. J. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748533</dc:identifier>
<dc:title><![CDATA[Loss of ELM1B impairs mitochondrial fission, matrix redox state and stress tolerance in Physcomitrium patens]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748622v1?rss=1">
<title>
<![CDATA[
Effects of spectral light quality on growth, photosynthetic pigments and bioactive compounds in Brassicaceae microgreens 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748622v1?rss=1
</link>
<description><![CDATA[
LED spectral composition is an important tool for improving the growth and nutritional quality of microgreens cultivated in controlled environments. This study evaluated the effects of three LED light treatments on growth, morphology, pigments, primary metabolites, phenolic composition, and antioxidant capacity in arugula (Eruca sativa), mustard (Brassica juncea), and radish (Raphanus sativus) microgreens. Microgreens were cultivated under controlled environmental conditions and exposed to broad-spectrum white (W), blue-enriched white (WB), and red-enriched white (R) light at a photosynthetic photon flux density of 200 micromol/m2/s. Light quality did not affect yield in any species. However, R increased cotyledon area in arugula by 50 to 60% and promoted hypocotyl elongation in both arugula and radish, whereas W resulted in the longest hypocotyls in mustard. Photosynthetic pigment composition responded differently among species. In mustard, WB increased the chlorophyll a/b ratio (1.12 to 1.18), whereas lutein concentration decreased from 7.06 to 4.20 mg 100 g/FW. Primary metabolism also responded to light treatments in a species-dependent manner. In mustard, W increased glucose (0.43 vs. 0.26 and 0.29 g 100 g/ FW) and fructose (0.33 vs. 0.20 and 0.22 g 100 g/ FW) concentrations compared with WB and R. Organic acid composition was more responsive to light treatments in radish, with higher concentrations under R. Phenolic metabolism also responded in a species-dependent manner. In mustard, W increased total phenolic content to 0.25 mg GAE g/FW compared with 0.15 mg GAE g/FW under WB and R, and ABTS antioxidant capacity to 1.17 mg TE g/FW compared with 0.74 and 0.75 mg TE g/FW under WB and R, respectively. Individual phenolic compounds were also affected by light treatments, particularly in arugula and mustard. These findings demonstrate that the effects of LED spectral composition on microgreen quality are highly species-dependent. Therefore, LED light spectra should be optimized according to the target species and the desired quality attributes rather than applying a single lighting strategy to all Brassicaceae microgreens.
]]></description>
<dc:creator><![CDATA[ Gonzalez, V., Quero, G. E., Riella, V., Zaccari, F., Silveira, A. C. ]]></dc:creator>
<dc:date>2026-09-03</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748622</dc:identifier>
<dc:title><![CDATA[Effects of spectral light quality on growth, photosynthetic pigments and bioactive compounds in Brassicaceae microgreens]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-03</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748336v1?rss=1">
<title>
<![CDATA[
A single dsRNA spray silences VAMT and shifts habanero pepper fruit metabolism towards capsinoids 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748336v1?rss=1
</link>
<description><![CDATA[
Capsaicinoids are synthesized in the placenta of Capsicum fruit, where vanillylamine aminotransferase (VAMT) catalyzes the formation of vanillylamine, the precursor of the pathway. The modulation of pungency has relied on genetic breeding and transgenic approaches, and this pathway has not been addressed by spray-induced gene silencing. The aim of this study was to evaluate whether a single non-invasive spray of double-stranded RNA (dsRNA) targeting VAMT allows the gene to be silenced and capsaicinoid accumulation to be modified in Capsicum chinense fruit. The molecule was designed in silico and applied at 10 days post-anthesis. Pedicel injection reduced the VAMT transcript in a dose-dependent manner, with three levels of inhibition distinguishable from one another. Spraying with surfactant reduced it by 86.2 %, a magnitude statistically indistinguishable from the 90.6 % obtained by injection, and also reduced the Pun1 transcript, a co-regulation previously described only as a difference between cultivars. Analysis by gas chromatography coupled to mass spectrometry showed reductions of 84.4 % in capsaicin and 68.5 % in dihydrocapsaicin, the loss of nonivamide and one further vanillylamine-derived compound, and the detection of capsiate and a second capsinoid, absent in control fruits. The siRNA was detected in non-treated tissues, and a single topical application is therefore sufficient to silence an endogenous biosynthetic gene and shift the metabolic profile of the fruit without genetic modification.
]]></description>
<dc:creator><![CDATA[ Arellano-Ordonez, E., Rico-Chavez, A. K., Torres-Pacheco, I., Ocampo-Velazquez, R. V., Guevara-Gonzalez, R. G., Cedillo, C. ]]></dc:creator>
<dc:date>2026-09-02</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748336</dc:identifier>
<dc:title><![CDATA[A single dsRNA spray silences VAMT and shifts habanero pepper fruit metabolism towards capsinoids]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-02</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748492v1?rss=1">
<title>
<![CDATA[
Impact of Water Deficit on Growth, Biochemical, and Physiological Traits in Eggplant MAGIC Lines 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748492v1?rss=1
</link>
<description><![CDATA[
Climate change exacerbates agricultural water scarcity, necessitating the development of drought-tolerant crop varieties. This study evaluates 12 eggplant lines from a MAGIC (Multi-parent Advanced Generation Intercross) population, previously selected for contrasting responses to water deficit during the vegetative stage. To validate tolerance under adult production conditions, plants underwent five irrigation-withholding cycles over a 170-day greenhouse growing period. Yield components, the Stress Tolerance Index (STI), and physiological parameters (water status and stomatal conductance) were evaluated. Additionally, photosynthetic pigments, oxidative stress markers, antioxidant compounds, and osmolytes were quantified to characterize the biochemical basis of tolerance alongside final biomass production. The results showed that four of the five lines that were previously classified as tolerant in the vegetative stage remained among the most tolerant at the reproductive stage. Specifically, lines L13, L78 and L179 were the most productive under water-limited conditions. While L13 and L179 exhibited stable tolerance throughout all developmental stages, L78 displayed stage-specific tolerance, manifested only during the reproductive growth phase. These findings emphasise the importance of integrating early-stage screening with adult-stage validation in order to capture the full spectrum of genetic drought tolerance. The most productive lines were characterised by moderate aboveground biomass, high leaf hydration and maintained stomatal conductance. However, the strategies employed differed: while L179 exhibited high photosynthetic pigment content, L13 was characterised by high total sugar accumulation. Overall, these results provide a multi-trait roadmap and identify elite MAGIC parental lines for breeding climate-resilient eggplant cultivars.
]]></description>
<dc:creator><![CDATA[ Flores-Saavedra, M., Plazas, M., Pacual-Seva, N., Vilanova, S., Vicente, O., Gramazio, P., Prohens, J. ]]></dc:creator>
<dc:date>2026-09-02</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748492</dc:identifier>
<dc:title><![CDATA[Impact of Water Deficit on Growth, Biochemical, and Physiological Traits in Eggplant MAGIC Lines]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-02</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.09.01.748490v1?rss=1">
<title>
<![CDATA[
Bioengineering of Pea (Pisum sativum) for the Expression of Myoglobin, a Heme-containing Animal Protein 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.09.01.748490v1?rss=1
</link>
<description><![CDATA[
Myoglobin, an oxygen-binding animal protein, was engineered in Pisum sativum (pea) to explore its potential as a food ingredient and balance the amino acid profile. In this study, minimal expression cassettes and binary vectors were used to express bovine myoglobin using particle gun and Agrobacterium-mediated transformation, respectively. Successful integration and expression of the myoglobin gene was achieved in P. sativum, with both methods yielding similar transformation efficiencies (~1%). Expression analysis of T2 seeds revealed that Agrobacterium-mediated transformation-derived transgenic lines that expressed myoglobin under the regulation of a Soybean 7S seed-specific promoter and Tobacco Etch Virus (TEV) translation enhancer and a chimeric Rb7MAR Terminator (Ps-BpRG13 events) consistently yielded the highest level of expression (0.32-1.57% of TSP), while transgenic lines with myoglobin expression under the regulation of a Soybean Phaseolin promoter and Rb7MAR Terminator (Ps-BpRG14 events) resulted in moderate levels of heterologous protein expression (0.13-0.83% TSP). Transgenic events with constitutive 2xCaMV35S promoter, TEV translation enhancer and Rb7MAR terminator (Ps-BpRG15 events) exhibited the lowest level of myoglobin expression (0.09-0.14% TSP). Co-bombardment of two minimal expression cassettes - one with myoglobin under the regulation of the Phaseolin promoter and Rb7MAR Terminator and the other with the nptII selectable marker under the regulation of a 2X constitutive CaMV35S promoter, TEV translational enhancer and TNOS Terminator, yielded lines that exhibited variable expression (0.03-0.77% TSP), with some events comparable in expression to Agrobacterium-derived Ps-pRG14 events. To the best of our knowledge, this is the first report of producing a heme-containing animal protein, myoglobin, in peas, with potential implications for sustainable production of food ingredients and nutritionally fortified and value-added plant products using molecular farming.
]]></description>
<dc:creator><![CDATA[ Ghogare, R., Williamson Benavides, B., Adams, K., Tarlyn, N., Clements, K. S., Velazquez, D., Wilcox, L., Holbrook, R., Helm, J., Grucan, N., Dhingra, A. ]]></dc:creator>
<dc:date>2026-09-02</dc:date>
<dc:identifier>doi:10.64898/2026.09.01.748490</dc:identifier>
<dc:title><![CDATA[Bioengineering of Pea (Pisum sativum) for the Expression of Myoglobin, a Heme-containing Animal Protein]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-02</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.31.748435v1?rss=1">
<title>
<![CDATA[
Arabidopsis Acyl-CoA Binding Protein 4, ACBP4, functions in developmentally programmed endoreduplication 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.31.748435v1?rss=1
</link>
<description><![CDATA[
Powdery mildew fungi induce localized endoreduplication, a variant of the cell cycle in which DNA is replicated but cells do not divide, in leaf mesophyll cells underlying the fungal feeding structure. Induced endoreduplication occurs concurrent with powdery mildew (PM) spore production and is associated with enhanced metabolic capacity and flux to lipids. The final ploidy of these cells is highly correlated with fungal spores produced and is the consequence of both basal (developmental) ploidy and PM-induced endoreduplication programs. Herein, we find the Arabidopsis lipid trafficking and regulatory protein ACYL-COA BINDING PROTEIN 4 (ACBP4) enhances PM spore production on Arabidopsis leaves. ACBP4 does not limit plant defense but instead supports basal mesophyll cell ploidy, with decreased final ploidy in cells underlying the fungal feeding structure in acbp4 mutants compared to wild-type (WT). Leaf epidermal cell size is decreased and stomatal density is increased in acbp4, consistent with a role for ACBP4 in developmentally programmed endoreduplication. Moreover, hypocotyl elongation in the dark, which is driven by programmed developmental endoreduplication, shows reduced hypocotyl length, cell length and ploidy in acbp4 versus WT. Together, our findings establish a novel means by which a plant ACBP promotes cell metabolism and development, with potential applications to agricultural productivity and quality.
]]></description>
<dc:creator><![CDATA[ Jaenisch, J., Tahmin, C. G., Wu, K. U., Wildermuth, M. C. ]]></dc:creator>
<dc:date>2026-09-02</dc:date>
<dc:identifier>doi:10.64898/2026.08.31.748435</dc:identifier>
<dc:title><![CDATA[Arabidopsis Acyl-CoA Binding Protein 4, ACBP4, functions in developmentally programmed endoreduplication]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-02</prism:publicationDate>
<prism:section></prism:section>
</item>
<item rdf:about="https://www.biorxiv.org/content/10.64898/2026.08.28.747744v1?rss=1">
<title>
<![CDATA[
A patient-derived LMX1B variant causes tissue-specific manifestations of nail-patella syndrome in mice 
]]>
</title>
<link>
https://www.biorxiv.org/content/10.64898/2026.08.28.747744v1?rss=1
</link>
<description><![CDATA[
Nail-patella syndrome (NPS) is a multisystem disorder caused by pathogenic variants in LMX1B and is characterized by dysplasia of the nails and patellae as well as extraskeletal complications such as progressive nephropathy and glaucoma. We generated a CRISPR/Cas9 knock-in mouse carrying the R252Q substitution, corresponding to a human LMX1B variant associated with renal-predominant disease. Phenotypic analysis revealed that homozygous mice were viable, but they displayed marked growth retardation and severe bilateral ocular opacity. Interestingly, while this model exhibited clear skeletal and ocular defects, the renal phenotype was relatively mild, although increased urinary albumin excretion, focal glomerular basement membrane abnormalities, and subtle changes in renal gene expression were detected. Beyond the classical NPS hallmarks, mutant mice also displayed midbrain morphological abnormalities, suggesting broader developmental consequences of this LMX1B variant. This patient-derived variant model not only recapitulates the pleiotropic features of NPS but also demonstrates organ-specific susceptibility to the R252Q substitution, providing a foundation for elucidating the complex molecular mechanisms underlying multisystem disease.
]]></description>
<dc:creator><![CDATA[ Amano, T., Yoshida, K., Sultana, F., Imura, C., Shiokawa, M., Shibuya, H., Takemura, K., Ikeda, K., Otsuka, C., Shinbo, K., Tamura, M., Mizuno, S. ]]></dc:creator>
<dc:date>2026-09-02</dc:date>
<dc:identifier>doi:10.64898/2026.08.28.747744</dc:identifier>
<dc:title><![CDATA[A patient-derived LMX1B variant causes tissue-specific manifestations of nail-patella syndrome in mice]]></dc:title>
<dc:publisher>Cold Spring Harbor Laboratory</dc:publisher>
<prism:publicationDate>2026-09-02</prism:publicationDate>
<prism:section></prism:section>
</item>
</rdf:RDF>
