bioRxiv Channel: <? echo $gname; ?> https://biorxiv.org This feed contains articles for bioRxiv Channel "" bioRxiv bioRxiv https://biorxiv.org <![CDATA[ Pediatric High Grade Glioma Resources From the Children’s Brain Tumor Tissue Consortium (CBTTC) and Pediatric Brain Tumor Atlas (PBTA) ]]> https://biorxiv.org/cgi/content/short/656587v1?rss=1" Ijaz, H. Koptyra, M. P. Gaonkar, K. S. Rokita, J. L. Baubet, V. P. Tauhid, L. Zhu, Y. Brown, M. A. Lopez Garcia, G. Zhang, B. Diskin, S. J. Vaksman, Z. Mason, J. L. Appert, E. M. Lilly, J. V. Lulla, R. R. De Raedt, T. Heath, A. P. Felmeister, A. Raman, P. Nazarian, J. Santi-Vicini, M. Storm, P. B. Resnick, A. C. Waanders, A. J. Cole, K. A. 2019-05-31 doi:10.1101/656587 Cold Spring Harbor Laboratory Press 2019-05-31 <![CDATA[ Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer ]]> https://biorxiv.org/cgi/content/short/704114v1?rss=1" Rashid, R. Gaglia, G. Chen, Y.-A. Lin, J.-R. Du, Z. Maliga, Z. Schapiro, D. Yapp, C. Muhlich, J. Sokolov, A. Sorger, P. Santagata, S. 2019-07-17 doi:10.1101/704114 Cold Spring Harbor Laboratory Press 2019-07-17 <![CDATA[ A quantitative framework for evaluating single-cell data structure preservation by dimensionality reduction techniques ]]> https://biorxiv.org/cgi/content/short/684340v1?rss=1" Heiser, C. N. Lau, K. S. 2019-06-27 doi:10.1101/684340 Cold Spring Harbor Laboratory Press 2019-06-27 <![CDATA[ Surface protein imputation from single cell transcriptomes by deep neural networks ]]> https://biorxiv.org/cgi/content/short/671180v1?rss=1" Zhou, Z. Wang, J. Ye, C. Zhang, N. 2019-06-14 doi:10.1101/671180 Cold Spring Harbor Laboratory Press 2019-06-14 <![CDATA[ Genetic Heterogeneity Profiling by Single Cell RNA Sequencing ]]> https://biorxiv.org/cgi/content/short/457622v1?rss=1" Zhou, Z. Zhang, N. R. 2018-10-30 doi:10.1101/457622 Cold Spring Harbor Laboratory Press 2018-10-30 <![CDATA[ scATAC-pro: a comprehensive workbench for single-cell chromatin accessibility sequencing data ]]> https://biorxiv.org/cgi/content/short/824326v1?rss=1" Yu, W. Uzun, Y. Zhu, Q. Chen, C. Tan, K. 2019-10-30 doi:10.1101/824326 Cold Spring Harbor Laboratory Press 2019-10-30 <![CDATA[ Predictive modeling of single-cell DNA methylome data enhances integration with transcriptome data ]]> https://biorxiv.org/cgi/content/short/2020.06.05.137000v1?rss=1" Uzun, Y. Wu, H. Tan, K. 2020-06-06 doi:10.1101/2020.06.05.137000 Cold Spring Harbor Laboratory Press 2020-06-06 <![CDATA[ Interpretative guides for interacting with tissue atlas and digital pathology data using the Minerva browser ]]> https://biorxiv.org/cgi/content/short/2020.03.27.001834v1?rss=1" Rashid, R. Chen, Y.-A. Hoffer, J. Muhlich, J. L. Lin, J.-R. Krueger, R. Pfister, H. Mitchell, R. Santagata, S. Sorger, P. 2020-03-30 doi:10.1101/2020.03.27.001834 Cold Spring Harbor Laboratory Press 2020-03-30 <![CDATA[ Accelerated single cell seeding in relapsed multiple myeloma ]]> https://biorxiv.org/cgi/content/short/2020.02.25.963272v1?rss=1" Landau, H. J. Yellapantula, V. Diamond, B. J. Rustad, E. H. Maclachlan, K. Gundem, G. Medina-Martinez, J. Arango Ossa, J. E. Levine, M. Zhou, Y. Rajya, K. L. Baez, P. Attiyeh, M. Makohon-Moore, A. Zhang, L. Boyle, E. E. Ashby, C. Blaney, P. Patel, M. Zhang, Y. Dogan, A. Chung, D. Giralt, S. Lahoud, O. B. Peled, J. Scordo, M. Shah, G. Hassoun, H. Korde, N. S. Lesokhin, A. M. Lu, S. Mailankody, S. Shah, U. A. Smith, E. Hultcrantz, M. L. Ulaner, G. van Rhee, F. Morgan, G. Landgren, O. Papaemmanuil, E. Iacobuzio-Donahue, C. A. Maura, F. 2020-02-26 doi:10.1101/2020.02.25.963272 Cold Spring Harbor Laboratory Press 2020-02-26 <![CDATA[ Minimal Barriers to Invasion During Human Colorectal Tumor Growth ]]> https://biorxiv.org/cgi/content/short/842203v1?rss=1" Ryser, M. D. Mallo, D. Hall, A. Hardman, T. King, L. M. Sorribes, I. C. Maley, C. C. Marks, J. R. Hwang, E. S. Shibata, D. 2019-11-14 doi:10.1101/842203 Cold Spring Harbor Laboratory Press 2019-11-14 <![CDATA[ The Evolution of Human Cancer Gene Duplications across Mammals ]]> https://biorxiv.org/cgi/content/short/2020.03.05.978965v1?rss=1" Tollis, M. Schneider-Utaka, A. K. Maley, C. C. 2020-03-05 doi:10.1101/2020.03.05.978965 Cold Spring Harbor Laboratory Press 2020-03-05 <![CDATA[ Unmasking the tissue microecology of ductal carcinoma in situ with deep learning ]]> https://biorxiv.org/cgi/content/short/812735v1?rss=1" Narayanan, P. L. Raza, S. E. A. Hall, A. Marks, J. R. King, L. West, R. B. Hernandez, L. Dowsett, M. Gusterson, B. Maley, C. Hwang, S. E. Yuan, Y. 2019-10-28 doi:10.1101/812735 Cold Spring Harbor Laboratory Press 2019-10-28 <![CDATA[ Diverse noncoding mutations contribute to deregulation of cis-regulatory landscape in pediatric cancers ]]> https://biorxiv.org/cgi/content/short/843102v1?rss=1" He, B. Gao, P. Ding, Y.-y. Chen, C.-h. Chen, G. Chen, C. Kim, H. Tasian, S. K. Hunger, S. P. Tan, K. 2019-11-15 doi:10.1101/843102 Cold Spring Harbor Laboratory Press 2019-11-15 <![CDATA[ Massively parallel, time-resolved single-cell RNA sequencing with scNT-Seq ]]> https://biorxiv.org/cgi/content/short/2019.12.19.882050v1?rss=1" Qiu, Q. Hu, P. Govek, K. W. Camara, P. G. Wu, H. 2019-12-20 doi:10.1101/2019.12.19.882050 Cold Spring Harbor Laboratory Press 2019-12-20 <![CDATA[ A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors ]]> https://biorxiv.org/cgi/content/short/761429v1?rss=1" Slyper, M. Porter, C. B. M. Ashenberg, O. Waldman, J. Drokhlyansky, E. Wakiro, I. Smillie, C. Smith-Rosario, G. Wu, J. Dionne, D. Vigneau, S. Jane-Valbuena, J. Napolitano, S. Su, M.-J. Patel, A. G. Karlstrom, A. Gritsch, S. Nomura, M. Waghray, A. Gohil, S. H. Tsankov, A. M. Jerby-Arnon, L. Cohen, O. Klughammer, J. Rosen, Y. Gould, J. Li, B. Nguyen, L. Wu, C. J. Izar, B. Haq, R. Hodi, F. S. Yoon, C. H. Hata, A. N. Baker, S. J. Suva, M. L. Bueno, R. Stover, E. H. Matulonis, U. A. Clay, M. R. Dyer, M. A. Collins, N. B. Wagle, N. Rotem, A. Johnson, B. E. 2019-09-12 doi:10.1101/761429 Cold Spring Harbor Laboratory Press 2019-09-12 <![CDATA[ Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems ]]> https://biorxiv.org/cgi/content/short/2020.05.13.094268v1?rss=1" Alon, S. Goodwin, D. Sinha, A. Wassie, A. Chen, F. Daugharthy, E. Bando, Y. Kajita, A. Xue, A. Marrett, K. Prior, R. Cui, Y. Payne, A. Yao, C.-C. Suk, H.-J. Wang, R. Yu, C.-C. Tillberg, P. Reginato, P. Pak, N. Liu, S. Punthambaker, S. Iyer, E. Kohman, R. Miller, J. Lein, E. Lako, A. Cullen, N. Rodig, S. Helvie, K. Abravanel, D. Wagle, N. Johnson, B. Klughammer, J. Slyper, M. Waldman, J. Jane-Valbuena, J. Rozenblatt-Rosen, O. Regev, A. Church, G. Marblestone, A. Boyden, E. S. 2020-05-15 doi:10.1101/2020.05.13.094268 Cold Spring Harbor Laboratory Press 2020-05-15 <![CDATA[ VISTA: Virtual ImmunoSTAining for pancreatic disease quantification in murine cohorts ]]> https://biorxiv.org/cgi/content/short/2020.04.01.020842v1?rss=1" Ternes, L. Huang, G. Lanciault, C. Thibault, G. Riggers, R. Gray, J. W. Muschler, J. Chang, Y. H. 2020-04-02 doi:10.1101/2020.04.01.020842 Cold Spring Harbor Laboratory Press 2020-04-02 <![CDATA[ RESTORE: Robust intEnSiTy nORmalization mEthod for Multiplexed Imaging ]]> https://biorxiv.org/cgi/content/short/792770v1?rss=1" Chang, Y. H. Chin, K. Thibault, G. Eng, J. Grace, L. Gray, J. W. 2019-10-03 doi:10.1101/792770 Cold Spring Harbor Laboratory Press 2019-10-03 <![CDATA[ Predicting Primary Site of Secondary Liver Cancer with a Neural Estimator of Metastatic Origin (NEMO) ]]> https://biorxiv.org/cgi/content/short/689828v1?rss=1" Schau, G. F. Burlingame, E. A. Thibault, G. Anekpuritanang, T. Wang, Y. Gray, J. W. Corless, C. Chang, Y. H. 2019-07-02 doi:10.1101/689828 Cold Spring Harbor Laboratory Press 2019-07-02 <![CDATA[ SHIFT: speedy histological-to-immunofluorescent translation of whole slide images enabled by deep learning ]]> https://biorxiv.org/cgi/content/short/730309v1?rss=1" Burlingame, E. A. McDonnell, M. Schau, G. F. Thibault, G. Lanciault, C. Morgan, T. Johnson, B. E. Corless, C. Gray, J. W. Chang, Y. H. 2019-08-15 doi:10.1101/730309 Cold Spring Harbor Laboratory Press 2019-08-15 <![CDATA[ A workflow for visualizing human cancer biopsies using large-format electron microscopy ]]> https://biorxiv.org/cgi/content/short/675371v1?rss=1" Riesterer, J. L. Lopez, C. S. Stempinski, E. S. Williams, M. Loftis, K. Stoltz, K. Thibault, G. Lanicault, C. Williams, T. Gray, J. W. 2019-06-19 doi:10.1101/675371 Cold Spring Harbor Laboratory Press 2019-06-19 <![CDATA[ Single-cell analysis of human lung epithelia reveals concomitant expression of the SARS-CoV-2 receptor ACE2 with multiple virus receptors and scavengers in alveolar type II cells ]]> https://biorxiv.org/cgi/content/short/2020.04.16.045617v1?rss=1" Han, G. Sinjab, A. Treekitkarnmongkol, W. Brennan, P. Hara, K. Chang, K. Bogatenkova, E. Sanchez-Espiridion, B. Behrens, C. Gao, B. Girard, L. Zhang, J. Sepesi, B. Cascone, T. Byers, L. Gibbons, D. L. Chen, J. Moghaddam, S. J. Ostrin, E. J. Fujimoto, J. Shay, J. Heymach, J. V. Minna, J. D. Dubinett, S. Scheet, P. A. Wistuba, I. I. Hill, E. Telesco, S. Stevenson, C. Spira, A. E. Wang, L. Kadara, H. 2020-04-17 doi:10.1101/2020.04.16.045617 Cold Spring Harbor Laboratory Press 2020-04-17 <![CDATA[ Coordinated cellular neighborhoods orchestrate antitumoral immunity at the colorectal cancer invasive front ]]> https://biorxiv.org/cgi/content/short/743989v1?rss=1" Schürch, C. M. Bhate, S. S. Barlow, G. L. Phillips, D. J. Noti, L. Zlobec, I. Chu, P. Black, S. Demeter, J. McIlwain, D. R. Samusik, N. Goltsev, Y. Nolan, G. P. 2019-08-24 doi:10.1101/743989 Cold Spring Harbor Laboratory Press 2019-08-24 <![CDATA[ Machine and deep learning single-cell segmentation and quantification of multi-dimensional tissue images ]]> https://biorxiv.org/cgi/content/short/790162v1?rss=1" McKinley, E. T. Roland, J. T. Franklin, J. L. Macedonia, M. C. Vega, P. N. Shin, S. Coffey, R. J. Lau, K. 2019-10-02 doi:10.1101/790162 Cold Spring Harbor Laboratory Press 2019-10-02 <![CDATA[ Clinically adaptable polymer enables simultaneous spatial analysis of colonic tissues and biofilms ]]> https://biorxiv.org/cgi/content/short/2020.04.15.030874v1?rss=1" Macedonia, M. C. Drewes, J. L. Markham, N. O. Simmons, A. J. Roland, J. T. Vega, P. N. Scurrah, C. R. Coffey, R. J. Shrubsole, M. J. Sears, C. L. Lau, K. S. 2020-04-17 doi:10.1101/2020.04.15.030874 Cold Spring Harbor Laboratory Press 2020-04-17 <![CDATA[ Dual indexed design of in-Drop single-cell RNA-seq libraries improves sequencing quality and throughput ]]> https://biorxiv.org/cgi/content/short/835488v1?rss=1" Southard-Smith, A. N. Simmons, A. J. Chen, B. Jones, A. L. Ramirez-Solano, M. A. Vega, P. N. Scurrah, C. R. Zhao, Y. Brenan, M. J. Xuan, J. Porter, E. B. Chen, X. Brenan, C. J. H. Liu, Q. Quigley, L. N. M. Lau, K. N. 2019-11-08 doi:10.1101/835488 Cold Spring Harbor Laboratory Press 2019-11-08 <![CDATA[ Commensal-derived succinate enhances tuft cell specification and suppresses ileal inflammation ]]> https://biorxiv.org/cgi/content/short/776724v1?rss=1" Banerjee, A. Herring, C. A. Kim, H. Chen, B. Simmons, A. J. Southard-Smith, A. N. White, J. R. Ramirez Solano, M. A. Scoville, E. A. Liu, Q. Washington, M. K. Lau, K. S. 2019-09-20 doi:10.1101/776724 Cold Spring Harbor Laboratory Press 2019-09-20 <![CDATA[ Single cell profiling reveals novel tumor and myeloid subpopulations in small cell lung cancer ]]> https://biorxiv.org/cgi/content/short/2020.12.01.406363v1?rss=1" Chan, J. M. Quintanal-Villalonga, A. Gao, V. Xie, Y. Allaj, V. Chaudhary, O. Masilionis, I. Egger, J. Chow, A. Walle, T. Mattar, M. Yarlagadda, D. V. Wang, J. L. Offin, M. Ciampricotti, M. Bhanot, U. K. Lai, W. V. Bott, M. J. Jones, D. R. Ruiz, A. Hollmann, T. Poirier, J. T. Nawy, T. Mazutis, L. Sen, T. Pe'er, D. Rudin, C. M. 2020-12-01 doi:10.1101/2020.12.01.406363 Cold Spring Harbor Laboratory Press 2020-12-01 <![CDATA[ Single-cell multi-omics reveals elevated plasticity and stem-cell-like blasts relevant to the poor prognosis of KMT2A-rearranged leukemia ]]> https://biorxiv.org/cgi/content/short/2020.12.06.413930v1?rss=1" Chen, C. Yu, W. Alikarami, F. Qiu, Q. Chen, C.-h. Flournoy, J. Gao, P. Uzun, Y. Fang, L. Yu, Y. Zhu, Q. Wang, K. Libbrecht, C. Felmeister, A. Rozich, I. Ding, Y.-y. Hunger, S. P. Wu, H. Brown, P. A. Guest, E. M. Barrett, D. M. Bernt, K. M. Tan, K. 2020-12-07 doi:10.1101/2020.12.06.413930 Cold Spring Harbor Laboratory Press 2020-12-07 <![CDATA[ An Integrated Clinical, Omic, and Image Atlas of an Evolving Metastatic Breast Cancer ]]> https://biorxiv.org/cgi/content/short/2020.12.03.408500v1?rss=1" Johnson, B. E. Creason, A. L. Stommel, J. M. Keck, J. Parmar, S. Betts, C. B. Blucher, A. Boniface, C. Bucher, E. Burlingame, E. A. Chin, K. Eng, J. Feiler, H. S. Kolodzie, A. Kong, B. Labrie, M. Leyshock, P. Mitri, S. Patterson, J. Riesterer, J. L. Sivagnanam, S. Sudar, D. Thibault, G. Zheng, C. Nan, X. Heiser, L. M. Spellman, P. T. Thomas, G. V. Demir, E. Chang, Y. H. Coussens, L. M. Guimaraes, A. R. Corless, C. Goecks, J. Bergan, R. Mitri, Z. Mills, G. B. Gray, J. W. 2020-12-03 doi:10.1101/2020.12.03.408500 Cold Spring Harbor Laboratory Press 2020-12-03 <![CDATA[ Single-cell characterization of subsolid and solid lesions in the lung adenocarcinoma spectrum ]]> https://biorxiv.org/cgi/content/short/2020.12.25.424416v1?rss=1" Yanagawa, J. Tran, L. M. Fung, E. Wallace, W. D. Prosper, A. E. Fishbein, G. A. Shea, C. Hong, R. Liu, B. Salehi-Rad, R. Deng, J. Z. Gower, A. Campbell, J. D. Mazzilli, S. A. Beane, J. Kadara, H. Lenburg, M. E. Spira, A. Aberle, D. R. Krysan, K. Dubinett, S. M. 2020-12-26 doi:10.1101/2020.12.25.424416 Cold Spring Harbor Laboratory Press 2020-12-26 <![CDATA[ Human colorectal pre-cancer atlas identifies distinct molecular programs underlying two major subclasses of pre-malignant tumors ]]> https://biorxiv.org/cgi/content/short/2021.01.11.426044v1?rss=1" Chen, B. McKinley, E. T. Simmons, A. J. Ramirez, M. A. Zhu, X. Southard-Smith, A. N. Markham, N. O. Sheng, Q. Drewes, J. Xu, Y. Heiser, C. N. Zhou, Y. Revetta, F. Berry, L. D. Zheng, W. Washington, M. K. Cai, Q. Sears, C. L. Goldenring, J. R. Franklin, J. L. Vandekar, S. Roland, J. T. Su, T. Huh, W. J. Liu, Q. Coffey, R. J. Shrubsole, M. J. Lau, K. 2021-01-13 doi:10.1101/2021.01.11.426044 Cold Spring Harbor Laboratory Press 2021-01-13 <![CDATA[ GLUER: integrative analysis of single-cell omics and imaging data by deep neural network ]]> https://biorxiv.org/cgi/content/short/2021.01.25.427845v1?rss=1" Peng, T. Chen, G. Tan, K. 2021-01-26 doi:10.1101/2021.01.25.427845 Cold Spring Harbor Laboratory Press 2021-01-26 <![CDATA[ MCMICRO: A scalable, modular image-processing pipeline for multiplexed tissue imaging ]]> https://biorxiv.org/cgi/content/short/2021.03.15.435473v1?rss=1" Schapiro, D. Sokolov, A. Yapp, C. Muhlich, J. L. Hess, J. Lin, J.-R. Chen, Y.-A. Nariya, M. K. Baker, G. J. Ruokonen, J. Maliga, Z. Jacobson, C. A. Farhi, S. L. Abbondanza, D. McKinley, E. T. Betts, C. Regev, A. Coffey, R. J. Coussens, L. M. Santagata, S. Sorger, P. K. 2021-03-16 doi:10.1101/2021.03.15.435473 Cold Spring Harbor Laboratory Press 2021-03-16 <![CDATA[ Comprehensive generation, visualization, and reporting of quality control metrics for single-cell RNA sequencing data ]]> https://biorxiv.org/cgi/content/short/2020.11.16.385328v1?rss=1" Hong, R. Koga, Y. Bandyadka, S. Leshchyk, A. Wang, Z. Alabdullatif, S. Wang, Y. Akavoor, V. Cao, X. Sarfraz, I. Jansen, F. Johnson, W. E. Yajima, M. Campbell, J. D. 2020-11-17 doi:10.1101/2020.11.16.385328 Cold Spring Harbor Laboratory Press 2020-11-17 <![CDATA[ Spatial drivers and pre-cancer populations collaborate with the microenvironment in untreated and chemo-resistant pancreatic cancer ]]> https://biorxiv.org/cgi/content/short/2021.01.13.426413v1?rss=1" Cui Zhou, D. Jayasinghe, R. G. Herndon, J. M. Storrs, E. Mo, C.-K. Wu, Y. Fulton, R. S. Wyczalkowski, M. A. Fronick, C. C. Fulton, L. A. Thammavong, L. Sato, K. Zhu, H. Sun, H. Wang, L.-B. Li, Y. Zuo, C. McMichael, J. F. Davies, S. R. Appelbaum, E. L. Robbins, K. J. Chasnoff, S. E. Yang, X. Liu, R. Reeb, A. N. Wendl, M. C. Oh, C. Serasanambati, M. Lal, P. Varghese, R. Mashl, J. R. Ponce, J. Terekhanova, N. V. Naser Al Deen, N. Yao, L. Wang, F. Chen, L. Schnaubelt, M. Puram, S. V. Kim, A. H. Song, S.-K. Shoghi, K. I. Ju, T. Hawkins, W. G. Chen, K. Ch 2021-01-14 doi:10.1101/2021.01.13.426413 Cold Spring Harbor Laboratory Press 2021-01-14 <![CDATA[ Transition to invasive breast cancer is associated with progressive changes in the structure and composition of tumor stroma ]]> https://biorxiv.org/cgi/content/short/2021.01.05.425362v1?rss=1" Risom, T. Glass, D. R. Liu, C. C. Rivero-Gutierrez, B. Baranski, A. McCaffrey, E. F. Greenwald, N. F. Kagel, A. C. Strand, S. H. Varma, S. Kong, A. Keren, L. Srivastava, S. Zhu, C. Khair, Z. Veis, D. J. Deschryver, K. Vennam, S. Maley, C. Hwang, E. S. Marks, J. R. Bendall, S. C. Colditz, G. A. West, R. B. Angelo, M. 2021-01-06 doi:10.1101/2021.01.05.425362 Cold Spring Harbor Laboratory Press 2021-01-06 <![CDATA[ Multicellular immune hubs and their organization in MMRd and MMRp colorectal cancer ]]> https://biorxiv.org/cgi/content/short/2021.01.30.426796v1?rss=1" Pelka, K. Hofree, M. Chen, J. Sarkizova, S. Pirl, J. D. Jorgji, V. Bejnood, A. Dionne, D. Ge, W. H. Xu, K. H. Chao, S. X. Zollinger, D. R. Lieb, D. J. Reeves, J. W. Fuhrman, C. A. Hoang, M. L. Delorey, T. Nguyen, L. T. Waldmann, J. A. Klapholz, M. Wakiro, I. Cohen, O. Smillie, C. S. Cuoco, M. S. Wu, J. Su, M.-j. Yeung, J. Vijaykumar, B. Magnuson, A. M. Asinovski, N. Moll, T. Goder-Reiser, M. N. Applebaum, A. S. Brais, L. K. DelloStritto, L. K. Denning, S. L. Phillips, S. T. Hill, E. K. Meehan, J. K. Frederick, D. T. Sharova, T. Kanodia, A. Todres, E. Z 2021-02-01 doi:10.1101/2021.01.30.426796 Cold Spring Harbor Laboratory Press 2021-02-01 <![CDATA[ Multiplexed 3D atlas of state transitions and immune interactions in colorectal cancer ]]> https://biorxiv.org/cgi/content/short/2021.03.31.437984v1?rss=1" Lin, J.-R. Wang, S. Coy, S. Tyler, M. A. Yapp, C. Chen, Y.-A. Heiser, C. N. Lau, K. Santagata, S. Sorger, P. K. 2021-04-02 doi:10.1101/2021.03.31.437984 Cold Spring Harbor Laboratory Press 2021-04-02 <![CDATA[ Automated quality control and cell identification of droplet-based single-cell data using dropkick ]]> https://biorxiv.org/cgi/content/short/2020.10.08.332288v1?rss=1" Heiser, C. N. Wang, V. M. Chen, B. Hughey, J. J. Lau, K. S. 2020-10-09 doi:10.1101/2020.10.08.332288 Cold Spring Harbor Laboratory Press 2020-10-09 <![CDATA[ Single-cell analyses reveal a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer ]]> https://biorxiv.org/cgi/content/short/2021.03.24.436532v1?rss=1" Becker, W. R. Nevins, S. A. Chen, D. C. Chiu, R. Horning, A. Laquindanum, R. Mills, M. Chaib, H. Ladabaum, U. Longacre, T. Shen, J. Esplin, E. D. Kundaje, A. Ford, J. M. Curtis, C. Snyder, M. P. Greenleaf, W. J. 2021-03-25 doi:10.1101/2021.03.24.436532 Cold Spring Harbor Laboratory Press 2021-03-25 <![CDATA[ The breast pre-cancer atlas illustrates the molecular and micro-environmental diversity of ductal carcinoma in situ ]]> https://biorxiv.org/cgi/content/short/2021.05.11.443641v1?rss=1" Nachmanson, D. Officer, A. Mori, H. Gordon, J. Evans, M. F. Steward, J. Yao, H. O'Keefe, T. Hasteh, F. Stein, G. S. Jepsen, K. Weaver, D. L. Hirst, G. Sprague, B. L. Esserman, L. J. Borowsky, A. D. Stein, J. L. Harismendy, O. 2021-05-12 doi:10.1101/2021.05.11.443641 Cold Spring Harbor Laboratory Press 2021-05-12 <![CDATA[ The spatial landscape of progression and immunoediting in primary melanoma at single cell resolution ]]> https://biorxiv.org/cgi/content/short/2021.05.23.445310v1?rss=1" Nirmal, A. J. Maliga, Z. Vallius, T. Quattrochi, B. Chen, A. A. Jacobson, C. A. Pelletier, R. J. Yapp, C. Arias-Camison, R. Chen, Y.-A. Lian, C. G. Murphy, G. F. Santagata, S. Sorger, P. K. 2021-05-23 doi:10.1101/2021.05.23.445310 Cold Spring Harbor Laboratory Press 2021-05-23 <![CDATA[ Stitching and registering highly multiplexed whole slide images of tissues and tumors using ASHLAR software ]]> https://biorxiv.org/cgi/content/short/2021.04.20.440625v1?rss=1" Muhlich, J. L. Chen, Y.-A. Russell, D. P. W. Sorger, P. K. 2021-04-21 doi:10.1101/2021.04.20.440625 Cold Spring Harbor Laboratory Press 2021-04-21 <![CDATA[ UnMICST: Deep learning with real augmentation for robust segmentation of highly multiplexed images of human tissues ]]> https://biorxiv.org/cgi/content/short/2021.04.02.438285v1?rss=1" Yapp, C. Novikov, E. Jang, W.-D. Chen, Y.-A. Cicconet, M. Maliga, Z. Jacobson, C. A. Wei, D. Santagata, S. Pfister, H. Sorger, P. K. 2021-04-04 doi:10.1101/2021.04.02.438285 Cold Spring Harbor Laboratory Press 2021-04-04 <![CDATA[ The Human Tumor Atlas Network (HTAN) Breast PreCancer Atlas: A multi-omic integrative analysis of ductal carcinoma in situ with clinical outcomes ]]> https://biorxiv.org/cgi/content/short/2021.06.16.448585v1?rss=1" Strand, S. H. Rivero-Gutierrez, B. Houlahan, K. E. Seoane, J. A. King, L. Risom, T. Simpson, L. A. Vennam, S. Khan, A. Cisneros, L. Hardman, T. Harmon, B. Couch, F. Gallagher, K. Kilgore, M. Rocque, G. B. DeMichele, A. King, T. McAuliffe, P. Nangia, J. Lee, J. Tseng, J. Storniolo, A. M. Thompson, A. Gupta, G. Burns, R. Veis, D. J. DeSchryver, K. Zhu, C. Matusiak, M. Wang, J. Zhu, S. X. Tappenden, J. Ding, D. Y. Zhang, D. Luo, J. Jiang, S. Varma, S. Anderson, L. Straub, C. Srivastava, S. Curtis, C. Tibshirani, R. Angelo, R. M. Hall, A. Owzar, K. Pol 2021-06-16 doi:10.1101/2021.06.16.448585 Cold Spring Harbor Laboratory Press 2021-06-16 <![CDATA[ SnFFPE-Seq: towards scalable single nucleus RNA-Seq of formalin-fixed paraffin-embedded (FFPE) tissue ]]> https://biorxiv.org/cgi/content/short/2022.08.25.505257v1?rss=1" Chung, H. Melnikov, A. McCabe, C. Drokhlyansky, E. Van Wittenberghe, N. Magee, E. M. Waldman, J. Spira, A. Chen, F. Mazzilli, S. Rozenblatt-Rosen, O. Regev, A. 2022-08-25 doi:10.1101/2022.08.25.505257 Cold Spring Harbor Laboratory Press 2022-08-25 <![CDATA[ Integrated Molecular Characterization of Intraductal Papillary Mucinous Neoplasms: An NCI Cancer Moonshot Precancer Atlas Pilot Project ]]> https://biorxiv.org/cgi/content/short/2022.10.14.512148v1?rss=1" Semaan, A. Bernard, V. Wong, J. Makino, Y. Swartzlander, D. Rajapakshe, K. I. Lee, J. Officer, A. Schmidt, C. M. Wu, H. H. Scaife, C. L. Affolter, K. E. Nachmanson, D. Firpo, M. A. Yip-Schneider, M. Lowy, A. M. Harismendy, O. Sen, S. Maitra, A. A. Jakubek, Y. A. Guerrero, P. A. 2022-10-18 doi:10.1101/2022.10.14.512148 Cold Spring Harbor Laboratory Press 2022-10-18 <![CDATA[ SlideCNA: Spatial copy number alteration detection from Slide-seq-like spatial transcriptomics data ]]> https://biorxiv.org/cgi/content/short/2022.11.25.517982v1?rss=1" Zhang, D. K. Segerstolpe, A. Slyper, M. Waldman, J. Murray, E. Cohen, O. Ashenberg, O. Abravanel, D. Jane-Valbuena, J. Mages, S. Lako, A. Helvie, K. Rozenblatt-Rosen, O. Rodig, S. Chen, F. Wagle, N. Regev, A. Klughammer, J. 2022-11-27 doi:10.1101/2022.11.25.517982 Cold Spring Harbor Laboratory Press 2022-11-27 <![CDATA[ SEG: Segmentation Evaluation in absence of Ground truth labels ]]> https://biorxiv.org/cgi/content/short/2023.02.23.529809v1?rss=1" Sims, Z. Strgar, L. Thirumalaisamy, D. Heussner, R. Thibault, G. Chang, Y. H. 2023-02-24 doi:10.1101/2023.02.23.529809 Cold Spring Harbor Laboratory Press 2023-02-24 <![CDATA[ Molecular cartography uncovers evolutionary and microenvironmental dynamics in sporadic colorectal tumors ]]> https://biorxiv.org/cgi/content/short/2023.03.09.530832v1?rss=1" Heiser, C. N. Simmons, A. J. Revetta, F. McKinley, E. T. Ramirez-Solano, M. A. Wang, J. Shao, J. Ayers, G. D. Wang, Y. Glass, S. E. Kaur, H. Rolong, A. Chen, B. Vega, P. N. Drewes, J. L. Saleh, N. Vandekar, S. Jones, A. L. Washington, M. K. Roland, J. T. Sears, C. L. Liu, Q. Shrubsole, M. J. Coffey, R. J. Lau, K. S. 2023-03-12 doi:10.1101/2023.03.09.530832 Cold Spring Harbor Laboratory Press 2023-03-12 <![CDATA[ Germline-mediated immunoediting sculpts breast cancer subtypes and metastatic proclivity ]]> https://biorxiv.org/cgi/content/short/2023.03.15.532870v1?rss=1" Houlahan, K. E. Khan, A. Greenwald, N. F. West, R. B. Angelo, M. Curtis, C. 2023-03-16 doi:10.1101/2023.03.15.532870 Cold Spring Harbor Laboratory Press 2023-03-16 <![CDATA[ Leptomeningeal anti-tumor immunity follows unique signaling principles ]]> https://biorxiv.org/cgi/content/short/2023.03.17.533041v1?rss=1" Remsik, J. Tong, X. Kunes, R. Z. Li, M. J. Osman, A. Chabot, K. Sener, U. Wilcox, J. A. Isakov, D. Snyder, J. Bale, T. Chaligne, R. Pe'er, D. D. Boire, A. 2023-03-20 doi:10.1101/2023.03.17.533041 Cold Spring Harbor Laboratory Press 2023-03-20 <![CDATA[ Inference of single cell profiles from histology stains with the Single-Cell omics from Histology Analysis Framework (SCHAF) ]]> https://biorxiv.org/cgi/content/short/2023.03.21.533680v1?rss=1" Comiter, C. Vaishnav, E. D. Ciapmricotti, M. Li, B. Yang, Y. Rodig, S. J. Turner, M. Pfaff, K. L. Jane-Valbuena, J. Slyper, M. Waldman, J. Vigneau, S. Wu, J. Blosser, T. R. Segerstolpe, A. Abravanel, D. Wagle, N. Zhuang, X. Rudin, C. M. Klughammer, J. Rozenblatt-Rosen, O. Kobayash-Kirschvink, K. J. Shu, J. Regev, A. 2023-03-23 doi:10.1101/2023.03.21.533680 Cold Spring Harbor Laboratory Press 2023-03-23 <![CDATA[ CRACD, a gatekeeper restricting proliferation, heterogeneity, and immune evasion of small cell lung cancer ]]> https://biorxiv.org/cgi/content/short/2023.02.15.528365v1?rss=1" Zhang, S. Kim, K.-B. Huang, Y. Kim, D.-W. Kim, B. Ko, K.-P. Zou, G. Zhang, J. Jun, S. Kirk, N. A. Hwang, Y. E. Ban, Y. H. Chan, J. M. Rudin, C. M. PARK, K.-S. Park, J.-I. 2023-02-15 doi:10.1101/2023.02.15.528365 Cold Spring Harbor Laboratory Press 2023-02-15 <![CDATA[ Addressing persistent challenges in digital image analysis of cancerous tissues ]]> https://biorxiv.org/cgi/content/short/2023.07.21.548450v1?rss=1" Prabhakaran, S. Yapp, C. Baker, G. J. Beyer, J. Chang, Y. H. Creason, A. L. Krueger, R. Muhlich, J. Patterson, N. H. Sidak, K. Sudar, D. Taylor, A. J. Ternes, L. Troidl, J. Yubin, X. Sokolov, A. Tyson, D. R. Participants of the Cell Imaging Hackathon 2022, 2023-07-24 doi:10.1101/2023.07.21.548450 Cold Spring Harbor Laboratory Press 2023-07-24 <![CDATA[ The relationship between diet, plasma glucose, and cancer prevalence across vertebrates ]]> https://biorxiv.org/cgi/content/short/2023.07.31.551378v1?rss=1" Kapsetaki, S. E. Basile, A. J. Compton, Z. T. Rupp, S. M. Duke, E. G. Boddy, A. M. Harrison, T. M. Sweazea, K. L. Maley, C. C. 2023-08-02 doi:10.1101/2023.07.31.551378 Cold Spring Harbor Laboratory Press 2023-08-02 <![CDATA[ A Masked Image Modeling Approach to Cyclic Immunofluorescence (CyCIF) Panel Reduction and Marker Imputation ]]> https://biorxiv.org/cgi/content/short/2023.05.10.540265v1?rss=1" Sims, Z. Chang, Y. H. 2023-05-13 doi:10.1101/2023.05.10.540265 Cold Spring Harbor Laboratory Press 2023-05-13 <![CDATA[ Signal recovery in single cell batch integration ]]> https://biorxiv.org/cgi/content/short/2023.05.05.539614v1?rss=1" Zhang, Z. Mathew, D. Lim, T. Huang, S. Wherry, E. J. Minn, A. J. Ma, Z. Zhang, N. R. 2023-05-08 doi:10.1101/2023.05.05.539614 Cold Spring Harbor Laboratory Press 2023-05-08 <![CDATA[ Multiplex imaging of localized prostate tumors reveals changes in mast cell type composition and spatial organization of AR-positive cells in the tumor microenvironment ]]> https://biorxiv.org/cgi/content/short/2023.08.18.553854v1?rss=1" Eksi, S. E. Ak, C. Sayar, Z. Thibault, G. Burlingame, E. Eng, J. Chitsazan, A. Adey, A. Boniface, C. Spellman, P. T. Thomas, G. V. Kopp, R. Demir, E. Chang, Y. H. Stavrinides, V. 2023-08-21 doi:10.1101/2023.08.18.553854 Cold Spring Harbor Laboratory Press 2023-08-21 <![CDATA[ Quantitative image analysis pipeline for detecting circulating hybrid cells in immunofluorescence images with human-level accuracy ]]> https://biorxiv.org/cgi/content/short/2023.08.24.554733v1?rss=1" 50,000) when using conventional enumeration methods. On the other hand, our proposed approach is unbiased to ROI size. The SVM classifier trained on the {beta}-VAE encodings achieved an F1 score of 0.80, matching the average performance of annotators. Our pipeline enables researchers to explore the role of CHCs in cancer progression and assess their potential as a clinical biomarker for metastasis. Further, we demonstrate that the pipeline can identify discrete cellular phenotypes among PBMCs, highlighting its utility beyond CHCs. ]]> Anderson, A. Theison, H. Baik, J. Gibbs, S. Wong, M. H. Chang, Y. H. 2023-08-26 doi:10.1101/2023.08.24.554733 Cold Spring Harbor Laboratory Press 2023-08-26 <![CDATA[ Longitudinal and multimodal auditing of tumor adaptation to CDK4/6 inhibitors in HR+ metastatic breast cancers ]]> https://biorxiv.org/cgi/content/short/2023.09.27.557464v1?rss=1" Creason, A. L. Egger, J. Watson, C. Sivagnanam, S. Chin, K. MacPherson, K. Lin, J.-R. Chen, Y.-A. Johnson, B. E. Feiler, H. S. Galipeau, D. Navin, N. E. Demir, E. Chang, Y. H. Corless, C. L. Mitri, Z. I. Thomas, G. Sorger, P. K. Adey, A. C. Coussens, L. M. Gray, J. W. Mills, G. B. Goecks, J. 2023-09-29 doi:10.1101/2023.09.27.557464 Cold Spring Harbor Laboratory Press 2023-09-29 <![CDATA[ Analysis of ductal carcinoma in situ by self-reported race reveals molecular differences related to outcome. ]]> https://biorxiv.org/cgi/content/short/2023.09.28.560000v1?rss=1" Strand, S. H. Houlahan, K. E. Branch, V. Lynch, T. Harmon, B. Couch, F. Gallagher, K. Kilgore, M. Wei, S. DeMichele, A. King, T. McAuliffe, P. Curtis, C. Owzar, K. Marks, J. R. Colditz, G. A. Hwang, E. S. West, R. B. 2023-10-02 doi:10.1101/2023.09.28.560000 Cold Spring Harbor Laboratory Press 2023-10-02 <![CDATA[ Matrix and analysis metadata standards (MAMS) to facilitate harmonization and reproducibility of single-cell data ]]> https://biorxiv.org/cgi/content/short/2023.03.06.531314v1?rss=1" Wang, Y. Sarfraz, I. Teh, W. K. Sokolov, A. Herb, B. R. Creasy, H. H. Virshup, I. Dries, R. Degatano, K. Mahurkar, A. Schnell, D. J. Madrigal, P. Hilton, J. Gehlenborg, N. Tickle, T. Campbell, J. D. 2023-03-07 doi:10.1101/2023.03.06.531314 Cold Spring Harbor Laboratory Press 2023-03-07 <![CDATA[ Temporal recording of mammalian development and precancer ]]> https://biorxiv.org/cgi/content/short/2023.12.18.572260v1?rss=1" Islam, M. Yang, Y. Simmons, A. J. Shah, V. M. Pavan, M. K. Xu, Y. Tasneem, N. Chen, Z. Trinh, L. T. Molina, P. Ramirez-Solano, M. A. Sadien, I. Dou, J. Chen, K. Magnuson, M. A. Rathmell, J. Macara, I. G. Winton, D. J. Liu, Q. Zafar, H. Kalhor, R. Church, G. M. Shrubsole, M. J. Coffey, R. J. Lau, K. 2023-12-19 doi:10.1101/2023.12.18.572260 Cold Spring Harbor Laboratory Press 2023-12-19 <![CDATA[ Multiplexed 3D Analysis of Cell Plasticity and Immune Niches in Melanoma ]]> https://biorxiv.org/cgi/content/short/2023.11.10.566670v1?rss=1" Yapp, C. Nirmal, A. J. Zhou, F. Y. Maliga, Z. Montero Llopis, P. Murphy, G. F. Lian, C. Danuser, G. Santagata, S. Sorger, P. K. 2023-11-15 doi:10.1101/2023.11.10.566670 Cold Spring Harbor Laboratory Press 2023-11-15 <![CDATA[ 2D and 3D multiplexed subcellular profiling of nuclear instability in human cancer ]]> https://biorxiv.org/cgi/content/short/2023.11.07.566063v1?rss=1" Coy, S. Cheng, B. Lee, J. S. Rashid, R. Browning, L. Xu, Y. Chakrabarty, S. S. Yapp, C. Chan, S. Tefft, J. B. Spektor, A. Ligon, K. L. Baker, G. J. Pellman, D. Sorger, P. K. Santagata, S. 2023-11-11 doi:10.1101/2023.11.07.566063 Cold Spring Harbor Laboratory Press 2023-11-11 <![CDATA[ Differential chromatin accessibility and transcriptional dynamics define breast cancer subtypes and their lineages ]]> https://biorxiv.org/cgi/content/short/2023.10.31.565031v1?rss=1" Iglesia, M. D. Jayasinghe, R. G. Chen, S. Terekhanova, N. V. Herndon, J. M. Storrs, E. Karpova, A. Zhou, D. C. Naser Al Deen, N. Shinkle, A. T. Lu, R. J.-H. Caravan, W. Houston, A. Zhao, Y. Sato, K. Lal, P. Street, C. Rodrigues, F. M. Southard-Smith, A. N. Targino da Costa, A. L. N. Zhu, H. Mo, C.-K. Crowson, L. Fulton, R. S. Wyczalkowski, M. A. Fronick, C. C. Fulton, L. A. Sun, H. Davies, S. R. Appelbaum, E. L. Chasnoff, S. E. Carmody, M. Brooks, C. Liu, R. Wendl, M. C. Oh, C. Bender, D. Cruchaga, C. Harari, O. Bredemeyer, A. Lavine, K. Bose, R. Marge 2023-11-02 doi:10.1101/2023.10.31.565031 Cold Spring Harbor Laboratory Press 2023-11-02 <![CDATA[ Tumor microenvironmental determinants of high-risk DCIS progression ]]> https://biorxiv.org/cgi/content/short/2023.12.01.569676v1?rss=1" 5cm in size with at least one additional high-risk feature: young age (<45 years), high nuclear grade, hormone receptor negativity, HER2 positivity, the presence of comedonecrosis, or a palpable mass. The tumor immune microenvironment was characterized using multiplex immunofluorescence to identify immune cells and their spatial relationships within the ducts and stroma. Gene copy number analysis and whole exome DNA sequencing identified the mutational burden and driver mutations, and quantitative whole-transcriptome/gene expression analyses were performed. There was no association between the percent of the DCIS genome characterized by copy number variants (CNAs) and recurrence events (DCIS or invasive). Mutations, especially missense mutations, in the breast cancer driver genes PIK3CA and TP53 were common in this high-risk DCIS cohort (47% of evaluated lesions). Tumor infiltrating lymphocyte (TIL) density was higher in DCIS lesions with TP53 mutations (p=0.0079) compared to wildtype lesions, but not in lesions with PIK3CA mutations (p=0.44). Immune infiltrates were negatively associated with hormone receptor status and positively associated with HER2 expression. High levels of CD3+CD8-T cells were associated with good outcomes with respect to any subsequent recurrence (DCIS or invasive cancer), whereas high levels of CD3+Foxp3+ Treg cells were associated with poor outcomes. Spatial proximity analyses of immune cells and tumor cells demonstrated that close proximity of T cells with tumor cells was associated with good outcomes with respect to any recurrence as well as invasive recurrences. Interestingly, we found that myoepithelial continuity (distance between myoepithelial cells surrounding the involved ducts) was significantly lower in DCIS lesions compared to normal tissue (p=0.0002) or to atypical ductal hyperplasia (p=0.011). Gene set enrichment analysis identified several immune pathways associated with low myoepithelial continuity and a low myoepithelial continuity score was associated with better outcomes, suggesting that gaps in the myoepithelial layer may allow access/interactions between immune infiltrates and tumor cells. Our study demonstrates the immune microenvironment of DCIS, in particular the spatial proximity of tumor cells and T cells, and myoepithelial continuity are important determinants for progression of disease. ]]> Glencer, A. Ramalingam, K. Schindler, N. Mori, H. Ghule, P. Lee, K. C. Nachmanson, D. Officer, A. Harismendy, O. Stein, J. L. Stein, G. Weaver, D. Yau, C. Hirst, G. L. Campbell, M. J. Esserman, L. J. Borowsky, A. D. 2023-12-04 doi:10.1101/2023.12.01.569676 Cold Spring Harbor Laboratory Press 2023-12-04 <![CDATA[ A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade glioma ]]> https://biorxiv.org/cgi/content/short/2024.03.06.583588v1?rss=1" Sussman, J. H. Oldridge, D. A. Yu, W. Chen, C.-H. Zellmer, A. M. Rong, J. Parvaresh-Rizi, A. Thadi, A. Xu, J. Bandyopadhyay, S. Sun, Y. Wu, D. Hunter, C. E. Brosius, S. Ahn, K. J. Baxter, A. E. Koptyra, M. P. Vanguri, R. McGrory, S. Resnick, A. C. Storm, P. B. Amankulor, N. M. Santi, M. Viaene, A. N. Zhang, N. De Raedt, T. Cole, K. Tan, K. 2024-03-08 doi:10.1101/2024.03.06.583588 Cold Spring Harbor Laboratory Press 2024-03-08 <![CDATA[ A cellular and spatial atlas of TP53-associated tissue remodeling in lung adenocarcinoma ]]> https://biorxiv.org/cgi/content/short/2023.06.28.546977v1?rss=1" Zhao, W. Kepecs, B. Mahadevan, N. R. Segerstolpe, A. Weirather, J. L. Besson, N. R. Giotti, B. Soong, B. Y. Li, C. Vigneau, S. Slyper, M. Wakiro, I. Jane-Valbuena, J. Ashenberg, O. Rotem, A. Bueno, R. Rozenblatt-Rosen, O. Pfaff, K. Rodig, S. Hata, A. N. Regev, A. Johnson, B. E. Tsankov, A. M. 2023-06-29 doi:10.1101/2023.06.28.546977 Cold Spring Harbor Laboratory Press 2023-06-29 <![CDATA[ A spatial cell atlas of neuroblastoma reveals developmental, epigenetic and spatial axis of tumor heterogeneity ]]> https://biorxiv.org/cgi/content/short/2024.01.07.574538v1?rss=1" Patel, A. G. Ashenberg, O. Collins, N. B. Segerstolpe, A. Jiang, S. Slyper, M. Huang, X. Caraccio, C. Jin, H. Sheppard, H. Xu, K. Chang, T.-C. Orr, B. A. Shirinifard, A. Chapple, R. H. Shen, A. Clay, M. R. Tatevossian, R. G. Reilly, C. Patel, J. Lupo, M. Cline, C. Dionne, D. Porter, C. B. M. Waldman, J. Bai, Y. Zhu, B. Barrera, I. Murray, E. Vigneau, S. Napolitano, S. Wakiro, I. Wu, J. Grimaldi, G. Dellostritto, L. Helvie, K. Rotem, A. Lako, A. Cullen, N. Pfaff, K. L. Karlstrom, A. Jane-Valbuena, J. Todres, E. Thorner, A. Geeleher, P. Rodig, S. J. 2024-01-07 doi:10.1101/2024.01.07.574538 Cold Spring Harbor Laboratory Press 2024-01-07