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https://biorxiv.org
https://biorxiv.org/cgi/content/short/001289v1?rss=1"
Eric LewitusIva KelavaAlex T KalinkaPavel TomancakWieland B Huttner2013-12-13doi:10.1101/001289Cold Spring Harbor Laboratory Press2013-12-13
https://biorxiv.org/cgi/content/short/008938v1?rss=1"
Helena JamborVineeth SurendranathAlex T. KalinkaPavel MejstrikStephan SaalfeldPavel Tomancak2014-09-09doi:10.1101/008938Cold Spring Harbor Laboratory Press2014-09-09
https://biorxiv.org/cgi/content/short/028308v1?rss=1"
13,000 protein coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here we present a genome-wide fosmid library of {approx}10,000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins we have created transgenic lines and for a total of 207 lines we have assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we can visualise many proteins at endogenous expression levels and find a large fraction of them localising to subcellular compartments. Using complementation tests we demonstrate that two-thirds of the tagged proteins are fully functional. Moreover, our clones also enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will enable systematic analysis of protein expression and localisation in various cellular and developmental contexts.
]]>Mihail SarovChritiane BarzHelena JamborMarco Y HeinChristopher SchmiedDana SucholdBettina StenderStephan JanoschVinay K.J. VikasR.T. KrisnanK. AishwaryaIrene R.S. FerreiraRadoslaw K. EjsmontKatja FinklSusanne HassePhilipp KaempferNicole PlewkaElisabeth VinisSiegfried SchloissnigElisabeth KnustVolker HartensteinMatthias MannMani RamaswamiK. VijayRaghavanPavel TomancakFrank Schnorrer2015-10-04doi:10.1101/028308Cold Spring Harbor Laboratory Press2015-10-04
https://biorxiv.org/cgi/content/short/045187v1?rss=1"
Johannes GirstmairAnne ZakrzewskiFrançois LaprazMette Handberg-ThorsagerPavel TomancakPeter Gabriel PitroneFraser SimpsonMaximilian J Telford2016-03-23doi:10.1101/045187Cold Spring Harbor Laboratory Press2016-03-23
https://biorxiv.org/cgi/content/short/112623v1?rss=1"
Wolff, C.Tinevez, J.-Y.Pietzsch, T.Stamataki, E.Harich, B.Preibisch, S.Shorte, S.Keller, P. J.Tomancak, P.Pavlopoulos, A.2017-02-28doi:10.1101/112623Cold Spring Harbor Laboratory Press2017-02-28
https://biorxiv.org/cgi/content/short/115220v1?rss=1"
Papadopoulos, D. K.Skouloudaki, K.Engstrom, Y.Terenius, L.Rigler, R.Vukojevic, V.Tomancak, P.2017-03-14doi:10.1101/115220Cold Spring Harbor Laboratory Press2017-03-14
https://biorxiv.org/cgi/content/short/117515v1?rss=1"
Viktorinova, I.Henry, I.Tomancak, P.2017-03-16doi:10.1101/117515Cold Spring Harbor Laboratory Press2017-03-16
https://biorxiv.org/cgi/content/short/000570v1?rss=1"
Helena KhaliullinaMesut BilginJulio L. SampaioAndrej ShevchenkoSuzanne Eaton2013-11-18doi:10.1101/000570Cold Spring Harbor Laboratory Press2013-11-18
https://biorxiv.org/cgi/content/short/002626v1?rss=1"
Jonathan RodenfelsOksana LavrynenkoSophie AyciriexJulio L SampaioMaria CarvalhoAndrej ShevchenkoSuzanne Eaton2014-02-14doi:10.1101/002626Cold Spring Harbor Laboratory Press2014-02-14
https://biorxiv.org/cgi/content/short/059923v1?rss=1"
Marko BrankatschkTheresia GutmannMichal GrzybekBeate BrankatschkUenal CoskunSuzanne Eaton2016-06-20doi:10.1101/059923Cold Spring Harbor Laboratory Press2016-06-20
https://biorxiv.org/cgi/content/short/140038v1?rss=1"
Dye, N. A.Popovic, M.Spannl, S.Etournay, R.Kainmueller, D.Myers, E. W.Julicher, F.Eaton, S.2017-05-19doi:10.1101/140038Cold Spring Harbor Laboratory Press2017-05-19
https://biorxiv.org/cgi/content/short/238634v1?rss=1"
Roscito, J. G.Sameith, K.Parra, G.Langer, B.Petzold, A.Rodrigues, M. T.Hiller, M.2017-12-22doi:10.1101/238634Cold Spring Harbor Laboratory Press2017-12-22
https://biorxiv.org/cgi/content/short/225722v1?rss=1"
Florio, M.Heide, M.Brandl, H.Pinson, A.Winkler, S.Wimberger, P.Huttner, W. B.Hiller, M.2017-11-28doi:10.1101/225722Cold Spring Harbor Laboratory Press2017-11-28
https://biorxiv.org/cgi/content/short/006916v1?rss=1"
Yusuke ToyodaCihan ErkutFrancisco Pan-MontojoSebastian BolandMartin P. StewartDaniel J. M?llerWolfgang WurstAnthony A. HymanTeymuras V. Kurzchalia2014-07-04doi:10.1101/006916Cold Spring Harbor Laboratory Press2014-07-04
https://biorxiv.org/cgi/content/short/067223v1?rss=1"
Oliver WuesekeDavid ZwickerAnne SchwagerYao Liang WongKaren OegemaFrank JulicherAnthony A HymanJeffrey Woodruff2016-08-01doi:10.1101/067223Cold Spring Harbor Laboratory Press2016-08-01
https://biorxiv.org/cgi/content/short/093054v1?rss=1"
Woodruff, J. B.Ferreira Gomes, B.Widlund, P. O.Mahamid, J.Hyman, A. A.2016-12-10doi:10.1101/093054Cold Spring Harbor Laboratory Press2016-12-10
https://biorxiv.org/cgi/content/short/096727v1?rss=1"
Leaver, M.Kayhan, M.McGaughran, A.Roedelsperger, C.Hyman, A. A.Sommer, R.2016-12-29doi:10.1101/096727Cold Spring Harbor Laboratory Press2016-12-29
https://biorxiv.org/cgi/content/short/119800v1?rss=1"
Hernandez-Vega, A.Braun, M.Scharrel, L.Jahnel, M.Wegmann, S.Hyman, B. T.Alberti, S.Diez, S.Hyman, A. A.2017-04-12doi:10.1101/119800Cold Spring Harbor Laboratory Press2017-04-12
https://biorxiv.org/cgi/content/short/200428v1?rss=1"
Hsu, F.Spannl, S.Ferguson, C.Hyman, A. A.Parton, R. G.Zerial, M.2017-10-09doi:10.1101/200428Cold Spring Harbor Laboratory Press2017-10-09
https://biorxiv.org/cgi/content/short/147918v1?rss=1"
Lehmann, M.Hebbar, S.Brandl, H.Leng, W.Lakshmanaperumal, N.Winkler, S.Knust, E.2017-06-09doi:10.1101/147918Cold Spring Harbor Laboratory Press2017-06-09
https://biorxiv.org/cgi/content/short/125716v1?rss=1"
Joseph, S.Palfy, M.Hilbert, L.Kumar, M.Karschau, J.Zaburdaev, V.Shevchenko, A.Vastenhouw, N.2017-04-13doi:10.1101/125716Cold Spring Harbor Laboratory Press2017-04-13
https://biorxiv.org/cgi/content/short/234112v1?rss=1"
Hilbert, L.Sato, Y.Kimura, H.Jülicher, F.Honigmann, A.Zaburdaev, V.Vastenhouw, N.2017-12-15doi:10.1101/234112Cold Spring Harbor Laboratory Press2017-12-15
https://biorxiv.org/cgi/content/short/174078v1?rss=1"
Decker, F.Oriola, D.Dalton, B.Brugues, J.2017-08-09doi:10.1101/174078Cold Spring Harbor Laboratory Press2017-08-09
https://biorxiv.org/cgi/content/short/177733v1?rss=1"
Berndt, F.Shah, G.Brugues, J.Huisken, J.2017-08-17doi:10.1101/177733Cold Spring Harbor Laboratory Press2017-08-17
https://biorxiv.org/cgi/content/short/003277v1?rss=1"
Ivana PetrovskaElisabeth NüskeMatthias C MunderGayathrie KulasegaranLiliana MalinovskaSonja KroschwaldDoris RichterKarim FahmyKimberley GibsonJean-Marc VerbavatzSimon Alberti2014-03-07doi:10.1101/003277Cold Spring Harbor Laboratory Press2014-03-07
https://biorxiv.org/cgi/content/short/076224v1?rss=1"
Matthias KaiserFlorian JugOlin SilanderSiddharth DeshpandeThomas PfohlThomas JulouGene MyersErik van Nimwegen2016-09-20doi:10.1101/076224Cold Spring Harbor Laboratory Press2016-09-20
https://biorxiv.org/cgi/content/short/066761v1?rss=1"
Jaroslav IchaChristiane GrunertMauricio Rocha-MartinsCaren Norden2016-07-29doi:10.1101/066761Cold Spring Harbor Laboratory Press2016-07-29
https://biorxiv.org/cgi/content/short/156943v1?rss=1"
Kumar, M.Joseph, S. R.Augsburg, M.Bogdanova, A.Drechsel, D.Vastenhouw, N. L.Buchholz, F.Gentzel, M.Shevchenko, A.2017-07-03doi:10.1101/156943Cold Spring Harbor Laboratory Press2017-07-03
https://biorxiv.org/cgi/content/short/143057v1?rss=1"
Weber, M.Scherf, N.Kohl, P.Huisken, J.2017-05-27doi:10.1101/143057Cold Spring Harbor Laboratory Press2017-05-27
https://biorxiv.org/cgi/content/short/173583v1?rss=1"
Shah, G.Thierbach, K.Schmid, B.Reade, A.Roeder, I.Scherf, N.Huisken, J.2017-08-08doi:10.1101/173583Cold Spring Harbor Laboratory Press2017-08-08
https://biorxiv.org/cgi/content/short/082958v1?rss=1"
Norden, C.Sidhaye, J.Ifflaender, N.2016-10-24doi:10.1101/082958Cold Spring Harbor Laboratory Press2016-10-24
https://biorxiv.org/cgi/content/short/236463v1?rss=1"
Weigert, M.Schmidt, U.Boothe, T.Muumlller, A.Dibrov, A.Jain, A.Wilhelm, B.Schmidt, D.Broaddus, C.Culley, S.Rocha-Martins, M.Segovia-Miranda, F.Norden, C.Henriques, R.Zerial, M.Solimena, M.Rink, J.Tomancak, P.Royer, L.Jug, F.Myers, E. W.2017-12-19doi:10.1101/236463Cold Spring Harbor Laboratory Press2017-12-19
https://biorxiv.org/cgi/content/short/106252v1?rss=1"
Tischler, G.Myers, E. W.2017-02-06doi:10.1101/106252Cold Spring Harbor Laboratory Press2017-02-06
https://biorxiv.org/cgi/content/short/145474v1?rss=1"
Tischler, G.2017-06-02doi:10.1101/145474Cold Spring Harbor Laboratory Press2017-06-02
https://biorxiv.org/cgi/content/short/263061v1?rss=1"
Cheeseman, B. L.Günther, U.Susik, M.Gonciarz, K.Sbalzarini, I. F.2018-02-09doi:10.1101/263061Cold Spring Harbor Laboratory Press2018-02-09
https://biorxiv.org/cgi/content/short/278937v1?rss=1"
Gao, M.Maraspini, R.Beutel, O.Zehtabian, A.Eickholt, B.Honigmann, A.Ewers, H.2018-03-08doi:10.1101/278937Cold Spring Harbor Laboratory Press2018-03-08
https://biorxiv.org/cgi/content/short/296566v1?rss=1"
nView larger version (36K):norg.highwire.dtl.DTLVardef@15b00edorg.highwire.dtl.DTLVardef@199eb2dorg.highwire.dtl.DTLVardef@1f0550org.highwire.dtl.DTLVardef@95cb5a_HPS_FORMAT_FIGEXP M_FIG C_FIG One sentence summaryFlows induced in living cell nuclei reveal the rheological changes that bring chromosomes under mechanical control during mitosis.
]]>Mittasch, M.Fritsch, A. W.Nestler, M.Iglesias-Artola, J. M.Subramanian, K.Petzold, H.Kar, M.Voigt, A.Kreysing, M.2018-04-07doi:10.1101/296566Cold Spring Harbor Laboratory Press2018-04-07
https://biorxiv.org/cgi/content/short/324822v1?rss=1"
Vu, H. T.-K.Mansour, S.Kuecken, M.Blasse, C.Basquin, C.Azimzadeh, J.Myers, E. W.Brusch, L.Rink, J. C.2018-05-17doi:10.1101/324822Cold Spring Harbor Laboratory Press2018-05-17
https://biorxiv.org/cgi/content/short/332916v1?rss=1"
Rink, J. C.Thommen, A.Werner, S.Frank, O.Philipp, J.Knittelfelder, O.Quek, Y.Fahmy, K.Shevchenko, A.Friedrich, B. M.Jülicher, F.2018-05-29doi:10.1101/332916Cold Spring Harbor Laboratory Press2018-05-29
https://biorxiv.org/cgi/content/short/395830v1?rss=1"
Kalebic, N.Gilardi, C.Albert, M.Namba, T.Long, K. R.Kostic, M.Langen, B.Huttner, W. B.2018-08-20doi:10.1101/395830Cold Spring Harbor Laboratory Press2018-08-20
https://biorxiv.org/cgi/content/short/495952v1?rss=1"
Morales-Navarrete, H.Nonaka, H.Scholich, A.Segovia-Miranda, F.de Back, W.Meyer, K.Bogorad, R. L.Koteliansky, V.Brusch, L.Kalaidzidis, Y.Julicher, F.Friedrich, B. M.Zerial, M.2018-12-13doi:10.1101/495952Cold Spring Harbor Laboratory Press2018-12-13
https://biorxiv.org/cgi/content/short/421701v1?rss=1"
Munster, S.Jain, A.Mietke, A.Pavlopoulos, A.Grill, S. W.Tomancak, P.2018-09-19doi:10.1101/421701Cold Spring Harbor Laboratory Press2018-09-19
https://biorxiv.org/cgi/content/short/532325v1?rss=1"
Papadopoulos, D. K.Tomancak, P.Tsarouhas, V.Samakovlis, C.Knust, E.Skouloudaki, K.2019-01-28doi:10.1101/532325Cold Spring Harbor Laboratory Press2019-01-28
https://biorxiv.org/cgi/content/short/524231v1?rss=1"
Oltsch, F.Klosin, A.Julicher, F.Hyman, A. A.Zechner, C.2019-01-22doi:10.1101/524231Cold Spring Harbor Laboratory Press2019-01-22
https://biorxiv.org/cgi/content/short/357186v1?rss=1"
Iyer, K. V.Piscitello-Gomez, R.Jülicher, F.Eaton, S.2018-06-27doi:10.1101/357186Cold Spring Harbor Laboratory Press2018-06-27
https://biorxiv.org/cgi/content/short/543066v1?rss=1"
Baumgart, J.Kirchner, M.Redemann, S.Woodruff, J.Verbavatz, J.-M.Julicher, F.Hyman, A.Mueller-Reichert, T.Brugues, J.2019-02-06doi:10.1101/543066Cold Spring Harbor Laboratory Press2019-02-06
https://biorxiv.org/cgi/content/short/499004v1?rss=1"
Goto-Silva, L.McShane, M. P.Salinas, S.Kalaidzidis, Y.Schiavo, G.Zerial, M.2018-12-17doi:10.1101/499004Cold Spring Harbor Laboratory Press2018-12-17
https://biorxiv.org/cgi/content/short/650762v1?rss=1"
Hansen, A. S.Zechner, C. S.2019-05-26doi:10.1101/650762Cold Spring Harbor Laboratory Press2019-05-26
https://biorxiv.org/cgi/content/short/711291v1?rss=1"
Schuhmacher, M.Grasskamp, A. T.Wagner, N.Lombardot, B.Schuhmacher, J. S.Sala, P.Lohmann, A.Henry, I.Shevchenko, A.Coskun, U.Walter, A. M.Nadler, A.2019-07-22doi:10.1101/711291Cold Spring Harbor Laboratory Press2019-07-22
https://biorxiv.org/cgi/content/short/721001v1?rss=1"
Wheeler, R. J.Lee, H. O.Poser, I.Pal, A.Doeleman, T.Kishigami, S.Kour, S.Anderson, E. N.Marrone, L.Murthy, A. C.Jahnel, M.Zhang, X.Boczek, E.Fritsch, A.Fawzi, N. L.Sterneckert, J.Pandey, U.David, D. C.Davis, B. G.Baldwin, A. J.Hermann, A.Bickle, M.Alberti, S.Hyman, A. A.2019-08-05doi:10.1101/721001Cold Spring Harbor Laboratory Press2019-08-05