bioRxiv Subject Collection: Synthetic Biology
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This feed contains articles for bioRxiv Subject Collection "Synthetic Biology"
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A generalizable system for antigenic peptide targeting across HLA-I allotypes
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https://www.biorxiv.org/content/10.64898/2026.05.21.726655v1?rss=1
2026-05-22doi:10.64898/2026.05.21.726655Cold Spring Harbor Laboratory2026-05-22
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High-throughput engineering of ligand-activated splicing ribozyme through domain insertion
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https://www.biorxiv.org/content/10.64898/2026.05.21.726912v1?rss=1
2026-05-22doi:10.64898/2026.05.21.726912Cold Spring Harbor Laboratory2026-05-22
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An Enhanced Split Intein-Mediated Ligation (SIML) Platform for Rapid Discovery and Functional Screening of Circular Bacteriocins
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https://www.biorxiv.org/content/10.64898/2026.05.21.726877v1?rss=1
2026-05-22doi:10.64898/2026.05.21.726877Cold Spring Harbor Laboratory2026-05-22
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3D bioprinting of engineered living materials in support slurries for complex free-standing structures
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https://www.biorxiv.org/content/10.64898/2026.05.20.726626v1?rss=1
8:1) and feature sizes as small as 260 m. The optimization of critical printing settings, including the ability of the bio-ink to retract during non-print movements, resulted in a reduction of unwanted bacterial deposition by nearly two orders of magnitude. Long-term viability experiments showed that bacteria in the bioprints could survive for at least 28 days with nutrient supplementation. Additionally, 3D-printed engineered biofilms revealed that incubation conditions and extracellular matrix composition significantly impacted the mechanical properties of printed constructs, with tradeoffs between matrix production and mechanical integrity. This study showcases an accessible 3D bioprinting platform for advanced bioprinting technologies, enabling development of engineered living materials with potential applications in synthetic biology, biotechnology, and tissue engineering.
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Clinical Campylobacter jejuni isolates: genomes and genetic tools
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https://www.biorxiv.org/content/10.64898/2026.05.21.726778v1?rss=1
2026-05-21doi:10.64898/2026.05.21.726778Cold Spring Harbor Laboratory2026-05-21
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A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints
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https://www.biorxiv.org/content/10.64898/2026.05.21.726110v1?rss=1
103). From these diversification libraries, we identified optimized variants including the anti-PD-L1 Dur1 with 2.01-fold enhanced binding, demonstrating the system's capacity for rapid antibody optimization from minimal diversity sampling. Collectively, ARESEC establishes a synthetic paradigm that transcends natural V(D)J constraints, generating multi-CDR diversification in non-lymphoid cells to enable rapid discovery of affinity-matured antibodies, effectively bridging immune evolution with modern antibody engineering demands.
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AI-assisted improvement of Aspergillus oryzae β-galactosidase using an Ensemble of Protein Language Models
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https://www.biorxiv.org/content/10.64898/2026.05.20.726739v1?rss=1
2026-05-21doi:10.64898/2026.05.20.726739Cold Spring Harbor Laboratory2026-05-21
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Quantitative Engineering and Investigation of Synthetic Sponge RNAs in E. coli
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https://www.biorxiv.org/content/10.64898/2026.05.19.726096v1?rss=1
2026-05-20doi:10.64898/2026.05.19.726096Cold Spring Harbor Laboratory2026-05-20
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Mechanism-informed rules tunably balance novelty and feasibility of predicted enzymatic reactions
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https://www.biorxiv.org/content/10.64898/2026.05.18.726002v1?rss=1
2026-05-19doi:10.64898/2026.05.18.726002Cold Spring Harbor Laboratory2026-05-19
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A Spectrum of Possibilities: A Systematic Evaluation of Fluorescent Proteins in Cyanobacteria
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https://www.biorxiv.org/content/10.64898/2026.05.18.725961v1?rss=1
2026-05-19doi:10.64898/2026.05.18.725961Cold Spring Harbor Laboratory2026-05-19
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Ratiometric transcriptional activation by protein degradation
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https://www.biorxiv.org/content/10.64898/2026.05.16.725679v1?rss=1
2026-05-18doi:10.64898/2026.05.16.725679Cold Spring Harbor Laboratory2026-05-18
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Repurposing native non-homologous end joining for multicopy random integration in Wickerhamomyces ciferrii
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https://www.biorxiv.org/content/10.64898/2026.05.17.725789v1?rss=1
2026-05-18doi:10.64898/2026.05.17.725789Cold Spring Harbor Laboratory2026-05-18
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Bioengineered algal lipids enriched in structured medium- and long-chain triacylglycerols, linoleate, and sn-2 palmitate for human milk fat substitutes
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https://www.biorxiv.org/content/10.64898/2026.05.15.725531v1?rss=1
2026-05-16doi:10.64898/2026.05.15.725531Cold Spring Harbor Laboratory2026-05-16
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Genetic code expansion enables programmable covalent protein design
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https://www.biorxiv.org/content/10.64898/2026.05.15.725538v1?rss=1
2026-05-16doi:10.64898/2026.05.15.725538Cold Spring Harbor Laboratory2026-05-16
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Heterologous expression of lyngbyatoxin biosynthetic genes in Aspergillus oryzae reveals transcriptional barriers but enables LtxC-mediated biotransformation
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https://www.biorxiv.org/content/10.64898/2026.05.15.725566v1?rss=1
2026-05-16doi:10.64898/2026.05.15.725566Cold Spring Harbor Laboratory2026-05-16
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Run or glide: muscles are indifferent while the tendon takes the strain
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https://www.biorxiv.org/content/10.64898/2026.05.15.725315v1?rss=1
2026-05-15doi:10.64898/2026.05.15.725315Cold Spring Harbor Laboratory2026-05-15
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HaloTag Ligand and HaloTag Protein engineering for a binary fluorescent turn-on probe
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https://www.biorxiv.org/content/10.64898/2026.05.15.724826v1?rss=1
2026-05-15doi:10.64898/2026.05.15.724826Cold Spring Harbor Laboratory2026-05-15
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Bacteriophage P22 virus-like particles as nanoscale protein scaffolds for plant synthetic biology
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https://www.biorxiv.org/content/10.64898/2026.05.14.725278v1?rss=1
2026-05-15doi:10.64898/2026.05.14.725278Cold Spring Harbor Laboratory2026-05-15
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Covalent tumor anchoring spatially orchestrates antitumor immunity
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https://www.biorxiv.org/content/10.64898/2026.05.13.724746v1?rss=1
2026-05-14doi:10.64898/2026.05.13.724746Cold Spring Harbor Laboratory2026-05-14
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Engineering high-titer lentiviral vectors for robust expression of RNA-based gene circuits
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https://www.biorxiv.org/content/10.64898/2026.05.12.724401v1?rss=1
2026-05-14doi:10.64898/2026.05.12.724401Cold Spring Harbor Laboratory2026-05-14
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Engineering orthogonal quorum sensing circuits using LuxR-type systems in yeast consortia
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https://www.biorxiv.org/content/10.64898/2026.05.13.724834v1?rss=1
2026-05-13doi:10.64898/2026.05.13.724834Cold Spring Harbor Laboratory2026-05-13
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Post-translational modification fidelity of recombinant human lactopontin expressed in Kluyveromyces lactis
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https://www.biorxiv.org/content/10.64898/2026.05.12.724256v1?rss=1
2026-05-12doi:10.64898/2026.05.12.724256Cold Spring Harbor Laboratory2026-05-12
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Programmable synthetic cytokine receptors polarize macrophages to user-defined functional states
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https://www.biorxiv.org/content/10.64898/2026.05.12.724672v1?rss=1
2026-05-12doi:10.64898/2026.05.12.724672Cold Spring Harbor Laboratory2026-05-12
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Engineered gut symbionts mediate cross-phylum antagonism to suppress uropathogenic Escherichia coli colonization
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https://www.biorxiv.org/content/10.64898/2026.05.11.724322v1?rss=1
2026-05-12doi:10.64898/2026.05.11.724322Cold Spring Harbor Laboratory2026-05-12
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DNA Staples: An oligonucleotide library for data storage and computing
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https://www.biorxiv.org/content/10.64898/2026.05.11.724305v1?rss=1
2026-05-12doi:10.64898/2026.05.11.724305Cold Spring Harbor Laboratory2026-05-12
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A two-step selection method for in vitro evolution of translational proteins
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https://www.biorxiv.org/content/10.64898/2026.05.09.724044v1?rss=1
2026-05-10doi:10.64898/2026.05.09.724044Cold Spring Harbor Laboratory2026-05-10
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Complete biosynthesis of penicillin G in Nicotiana benthamiana
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https://www.biorxiv.org/content/10.64898/2026.05.08.723852v1?rss=1
2026-05-10doi:10.64898/2026.05.08.723852Cold Spring Harbor Laboratory2026-05-10
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Phage-assisted continuous evolution of enzymes for noncanonical tyrosine biosynthesis
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https://www.biorxiv.org/content/10.64898/2026.05.08.723366v1?rss=1
2026-05-09doi:10.64898/2026.05.08.723366Cold Spring Harbor Laboratory2026-05-09
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Noise analysis of derivative-action biomolecular topologies
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https://www.biorxiv.org/content/10.64898/2026.05.06.723344v1?rss=1
2026-05-08doi:10.64898/2026.05.06.723344Cold Spring Harbor Laboratory2026-05-08
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FASTOP - Fast editing toolkit for top expression sites in yeast
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https://www.biorxiv.org/content/10.64898/2026.05.07.723299v1?rss=1
2026-05-08doi:10.64898/2026.05.07.723299Cold Spring Harbor Laboratory2026-05-08