bioRxiv Subject Collection: Synthetic Biology
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This feed contains articles for bioRxiv Subject Collection "Synthetic Biology"
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Biocontainment attenuation of mobile DNA host range in a wastewater microbiome
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https://www.biorxiv.org/content/10.64898/2026.07.13.738295v1?rss=1
100-fold and yielded mutations in 85% of the reads. Together, these findings reveal how pairs of plasmids expressing orthogonal cat-RNA can be used to monitor the effects of plasmid-encoded traits on mobile DNA persistence following HGT. They also highlight challenges when using biocontainment systems containing genes related to those found in the microbiomes targeted for engineering.
]]>2026-07-14doi:10.64898/2026.07.13.738295Cold Spring Harbor Laboratory2026-07-14
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Minimal Data, Maximal Insight (MDMI): A Structure-guided Pipeline for Discovering Functional Alternatives in Peptide-Protein Interfaces
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https://www.biorxiv.org/content/10.64898/2026.07.13.737974v1?rss=1
2026-07-14doi:10.64898/2026.07.13.737974Cold Spring Harbor Laboratory2026-07-14
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Programmable and Dynamic DNA Localisation at Synthetic Cell Membranes
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https://www.biorxiv.org/content/10.64898/2026.07.13.738173v1?rss=1
2026-07-14doi:10.64898/2026.07.13.738173Cold Spring Harbor Laboratory2026-07-14
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Engineering a flexible loop in S-adenosyl-L-methionine synthetase enables production of SAM nucleobase analogues with selective biochemical and cellular activity
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https://www.biorxiv.org/content/10.64898/2026.07.10.737877v1?rss=1
2026-07-13doi:10.64898/2026.07.10.737877Cold Spring Harbor Laboratory2026-07-13
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ARCHIVE: Machine-Guided Design of an Efficient Open-Ended DNA Recording Device to Increase Resolution of Multiplexed Cell History Tracking
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https://www.biorxiv.org/content/10.64898/2026.07.10.737758v1?rss=1
2026-07-13doi:10.64898/2026.07.10.737758Cold Spring Harbor Laboratory2026-07-13
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Air-driven aldehyde synthesis in engineered bacteria via gene deletion and aryl-alcohol oxidase profiling
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https://www.biorxiv.org/content/10.64898/2026.07.12.738062v1?rss=1
2026-07-13doi:10.64898/2026.07.12.738062Cold Spring Harbor Laboratory2026-07-13
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A microbial growth-coupled platform for in vivo interrogation of Rubisco oxygenase activity
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https://www.biorxiv.org/content/10.64898/2026.07.10.737725v1?rss=1
2026-07-10doi:10.64898/2026.07.10.737725Cold Spring Harbor Laboratory2026-07-10
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BxbI-mediated insertion of a 77kb human RET sensitive haplotype into the mouse genome to generate a humanized model of Hirschsprung disease
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https://www.biorxiv.org/content/10.64898/2026.07.05.736620v1?rss=1
2026-07-10doi:10.64898/2026.07.05.736620Cold Spring Harbor Laboratory2026-07-10
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Extracellular injection system combined with peptides for intracellular Staphylococcus aureus treatment
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https://www.biorxiv.org/content/10.64898/2026.07.06.736670v1?rss=1
2026-07-10doi:10.64898/2026.07.06.736670Cold Spring Harbor Laboratory2026-07-10
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MozClo: An Expanded MoClo Toolset for Large Multigene Assembly and Plant Transformations
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https://www.biorxiv.org/content/10.64898/2026.07.09.737387v1?rss=1
2026-07-10doi:10.64898/2026.07.09.737387Cold Spring Harbor Laboratory2026-07-10
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Transcription-induced coacervation accelerates and sensitizes cell-free biosensing
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https://www.biorxiv.org/content/10.64898/2026.07.02.736143v1?rss=1
2026-07-10doi:10.64898/2026.07.02.736143Cold Spring Harbor Laboratory2026-07-10
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Deoxyribonucleotide incorporation reshapes mRNA design beyond canonical ribonucleotide boundaries
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https://www.biorxiv.org/content/10.64898/2026.07.09.737403v1?rss=1
2026-07-10doi:10.64898/2026.07.09.737403Cold Spring Harbor Laboratory2026-07-10
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Fabrication and Use of a 32-Well LED-Embedded Microplate for Optogenetic Dynamic Control
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https://www.biorxiv.org/content/10.64898/2026.07.08.737360v1?rss=1
2026-07-10doi:10.64898/2026.07.08.737360Cold Spring Harbor Laboratory2026-07-10
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An Unusual Follower Peptide is Required for Biosynthesis of the Antibiotic Lasso Peptide Triculamin
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https://www.biorxiv.org/content/10.64898/2026.07.03.736388v1?rss=1
2026-07-10doi:10.64898/2026.07.03.736388Cold Spring Harbor Laboratory2026-07-10
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AAV delivered lysosome-targeting chimeras mediate sustained antibody depletion in vivo
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https://www.biorxiv.org/content/10.64898/2026.07.05.736665v1?rss=1
2026-07-10doi:10.64898/2026.07.05.736665Cold Spring Harbor Laboratory2026-07-10
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Domain Insertion Improves the Precision of a CRISPR Adenine Base Editor
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https://www.biorxiv.org/content/10.64898/2026.07.03.736350v1?rss=1
2026-07-10doi:10.64898/2026.07.03.736350Cold Spring Harbor Laboratory2026-07-10
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Engineered probiotic Escherichia coli-mediated intestinal nicotine clearance alleviates nonalcoholic steatohepatitis in mice
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https://www.biorxiv.org/content/10.64898/2026.07.02.736048v1?rss=1
2026-07-09doi:10.64898/2026.07.02.736048Cold Spring Harbor Laboratory2026-07-09
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Directed evolution of compact synthetic promoters via AlphaGenome and genetic algorithms
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https://www.biorxiv.org/content/10.64898/2026.06.28.735069v1?rss=1
2026-07-09doi:10.64898/2026.06.28.735069Cold Spring Harbor Laboratory2026-07-09
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Expanding all-α-helical protein space through rational computational design
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https://www.biorxiv.org/content/10.64898/2026.07.03.736327v1?rss=1
30% yield X-ray crystal structures matching the designs to atomic accuracy and with new-to-nature structures. Finally, several of the scaffolds are functionalised through one-shot designs to deliver ion, small-molecule and protein binders.
]]>2026-07-09doi:10.64898/2026.07.03.736327Cold Spring Harbor Laboratory2026-07-09
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A Chemically Defined Synthetic Cell Capable Of Growth And Replication
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https://www.biorxiv.org/content/10.64898/2026.07.01.735724v1?rss=1
2026-07-09doi:10.64898/2026.07.01.735724Cold Spring Harbor Laboratory2026-07-09
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Linker-Length Landscape Mapping Enables Coupling of Diverse Synthetic Chemically Induced Dimerization Systems to Molecular Readouts
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https://www.biorxiv.org/content/10.64898/2026.07.01.735888v1?rss=1
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Photoswitchable optoGPCRs for reversible control of Gs and arrestin signalling
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https://www.biorxiv.org/content/10.64898/2026.07.01.735182v1?rss=1
2026-07-09doi:10.64898/2026.07.01.735182Cold Spring Harbor Laboratory2026-07-09
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Crowdsourced riboregulators reveal design principles for programmable RNA switching
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https://www.biorxiv.org/content/10.64898/2026.07.08.737257v1?rss=1
2026-07-09doi:10.64898/2026.07.08.737257Cold Spring Harbor Laboratory2026-07-09
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Targeted DNA nicking enables efficient, single-step and counterselection-free editing of bacteriophage genomes
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https://www.biorxiv.org/content/10.64898/2026.06.25.734514v1?rss=1
2026-07-09doi:10.64898/2026.06.25.734514Cold Spring Harbor Laboratory2026-07-09
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Impact of coding region accessibility and uridine chemistry on translation in mRNA-DNA hybrid origami
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https://www.biorxiv.org/content/10.64898/2026.06.25.734245v1?rss=1
View larger version (36K):
org.highwire.dtl.DTLVardef@1240269org.highwire.dtl.DTLVardef@11a423corg.highwire.dtl.DTLVardef@f28d67org.highwire.dtl.DTLVardef@217409_HPS_FORMAT_FIGEXP M_FIG C_FIG
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Synergistic CRISPR-Cas Antimicrobials through Essential and Defensive Gene Cotargeting in Staphylococcus aureus
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https://www.biorxiv.org/content/10.64898/2026.06.25.734632v1?rss=1
2026-07-09doi:10.64898/2026.06.25.734632Cold Spring Harbor Laboratory2026-07-09
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LUstiGE, Light responsive Ustilago maydis Gene Expression: Optogenetic control of morphogenesis and pathogenesis in the corn fungal pathogen Ustilago maydis
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https://www.biorxiv.org/content/10.64898/2026.06.25.734638v1?rss=1
2026-07-09doi:10.64898/2026.06.25.734638Cold Spring Harbor Laboratory2026-07-09
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Complete elucidation and heterologous reconstruction of the biosynthetic pathway of camptothecin
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https://www.biorxiv.org/content/10.64898/2026.06.23.733941v1?rss=1
2026-07-08doi:10.64898/2026.06.23.733941Cold Spring Harbor Laboratory2026-07-08
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3' Exonuclease-mediated DNA assembly at room temperature and below
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https://www.biorxiv.org/content/10.64898/2026.06.17.732819v1?rss=1
2026-07-08doi:10.64898/2026.06.17.732819Cold Spring Harbor Laboratory2026-07-08
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Generative Drug Design in a Loop with dtSFM
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https://www.biorxiv.org/content/10.64898/2026.06.10.731501v1?rss=1
2026-07-08doi:10.64898/2026.06.10.731501Cold Spring Harbor Laboratory2026-07-08