bioRxiv Subject Collection: Biochemistry
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This feed contains articles for bioRxiv Subject Collection "Biochemistry"
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Iron exposure reprograms redox homeostasis through mitochondrial depolarization in neuroblastoma
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https://www.biorxiv.org/content/10.64898/2026.10.08.755735v1?rss=1
2026-10-09doi:10.64898/2026.10.08.755735Cold Spring Harbor Laboratory2026-10-09
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A glycosaminoglycan degrading Polysaccharide Utilization Locus in the oral microbiota species Segatella oris
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https://www.biorxiv.org/content/10.64898/2026.10.07.757250v1?rss=1
2026-10-09doi:10.64898/2026.10.07.757250Cold Spring Harbor Laboratory2026-10-09
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De novo protein design in an expanded chemical space
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https://www.biorxiv.org/content/10.64898/2026.10.08.757794v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757794Cold Spring Harbor Laboratory2026-10-09
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Purification-free cryo-EM reveals endogenous assemblies from minute tardigrade samples
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https://www.biorxiv.org/content/10.64898/2026.10.08.757488v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757488Cold Spring Harbor Laboratory2026-10-09
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Systematic Analysis of GARP- and EARP-Mediated Trafficking Pathways Using Lysosomal Proteomics
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https://www.biorxiv.org/content/10.64898/2026.10.08.757561v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757561Cold Spring Harbor Laboratory2026-10-09
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Identification of active deubiquitinases in dendritic cells under unstimulated and activated conditions.
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https://www.biorxiv.org/content/10.64898/2026.10.08.757166v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757166Cold Spring Harbor Laboratory2026-10-09
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A modular mechanism determines the proton-pumping stoichiometry of respiratory complex I
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https://www.biorxiv.org/content/10.64898/2026.10.08.757549v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757549Cold Spring Harbor Laboratory2026-10-09
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CLN6 is a glycerophosphoglycerol epimerase in bis(monoacylglycero)phosphate biosynthesis
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https://www.biorxiv.org/content/10.64898/2026.10.07.757350v1?rss=1
2026-10-09doi:10.64898/2026.10.07.757350Cold Spring Harbor Laboratory2026-10-09
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Defense-associated reverse transcriptases synthesize DNA in a telomerase-like manner
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https://www.biorxiv.org/content/10.64898/2026.10.08.757509v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757509Cold Spring Harbor Laboratory2026-10-09
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Mechanistic Basis of Sulfo Transfer in a PAPS-Independent ArylSulfate SulfoTransferases Revealed by Crystallography and QM/MM Simulations
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https://www.biorxiv.org/content/10.64898/2026.10.08.757636v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757636Cold Spring Harbor Laboratory2026-10-09
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Aging Differentially Remodels the Retina and RPE/Choroid Proteome and Metabolism
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https://www.biorxiv.org/content/10.64898/2026.10.07.757409v1?rss=1
2026-10-09doi:10.64898/2026.10.07.757409Cold Spring Harbor Laboratory2026-10-09
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A Practical Assay for Assessing Mean Red Blood Cell Age Using the Protein 4.1a/4.1b Ratio
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https://www.biorxiv.org/content/10.64898/2026.10.08.757720v1?rss=1
50 years, and males (P=0.151). The ratio showed modest positive correlations with chronological age in females (Spearman p=0.26, P=0.047) and males (p=0.33, P=0.015), while substantial interindividual variability remained across the age range. CONCLUSIONS: Direct isolation of RBC membranes from small volumes of whole blood enables reproducible determination of the protein 4.1a/4.1b ratio using a simplified workflow. Application of the assay to a comparatively large human population revealed substantial interindividual variation in this molecular marker of mean RBC age that was not explained by sex or chronological age alone. The method provides a practical approach for investigating biological variation in mean RBC age and its potential contribution to laboratory measurements influenced by RBC survival.
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Single-molecule reaction chambers reveal intramolecular protein kinase activation
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https://www.biorxiv.org/content/10.64898/2026.10.08.757607v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757607Cold Spring Harbor Laboratory2026-10-09
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Cancer-associated mutations reconfigure dynamical responses in EGFR kinase
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https://www.biorxiv.org/content/10.64898/2026.10.08.757680v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757680Cold Spring Harbor Laboratory2026-10-09
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The Batten disease gene product CLN6 is a glycerophosphoglycerol epimerase that establishes S,S-BMP stereochemistry
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https://www.biorxiv.org/content/10.64898/2026.10.07.757477v1?rss=1
2026-10-09doi:10.64898/2026.10.07.757477Cold Spring Harbor Laboratory2026-10-09
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Repurposing dopamine D3 receptor fluorescent ligands for D2 receptor
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https://www.biorxiv.org/content/10.64898/2026.10.07.756687v1?rss=1
2026-10-09doi:10.64898/2026.10.07.756687Cold Spring Harbor Laboratory2026-10-09
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Photobiocatalytic Diastereo- and Enantioselective Synthesis of Non-Canonical Amino Acids Bearing Challenging Stereochemical Dyads and Triads
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https://www.biorxiv.org/content/10.64898/2026.10.07.757362v1?rss=1
99:1 e.r.. To further demonstrate the synthetic utility of this platform, the stereochemically enriched amino acids were transformed into valuable {gamma}-chiral amines through engineered PLP dependent tryptophan decarboxylases, establishing a dual PLP enzyme based biocatalytic cascade for the enantiodivergent synthesis of privileged amine scaffolds. Molecular dynamics simulations reveal that enzyme controlled {gamma}-stereoselectivity originates from active-site remodeling that modulates {pi}-{pi} interactions and steric contacts governing the facial selectivity of the radical addition step. This work expands the synthetic capabilities of radical biocatalysis by enabling catalyst-controlled stereodivergent assembly of complex acyclic stereochemical arrays and establishes engineered PLP enzymes as versatile catalysts for the synthesis of stereochemically complex amino acids and chiral amines.
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Exploring the substrate specificity and catalytic mechanism in a PAPS independent ASST from Salmonella typhimurium
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https://www.biorxiv.org/content/10.64898/2026.10.08.757629v1?rss=1
2026-10-09doi:10.64898/2026.10.08.757629Cold Spring Harbor Laboratory2026-10-09
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Programming N-linked glycan composition through de novo protein design
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https://www.biorxiv.org/content/10.64898/2026.10.07.757460v1?rss=1
2026-10-08doi:10.64898/2026.10.07.757460Cold Spring Harbor Laboratory2026-10-08
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AI agent supervision of structural model building and refinement
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https://www.biorxiv.org/content/10.64898/2026.10.05.756902v1?rss=1
2026-10-08doi:10.64898/2026.10.05.756902Cold Spring Harbor Laboratory2026-10-08
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Structure-based virtual screening reveals new metabolite interactions and modulators of human glucose-6-phosphate isomerase
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https://www.biorxiv.org/content/10.64898/2026.10.06.757072v1?rss=1
2026-10-07doi:10.64898/2026.10.06.757072Cold Spring Harbor Laboratory2026-10-07
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Informatics Methods to Enhance Bacterial Peptide Detection
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https://www.biorxiv.org/content/10.64898/2026.10.06.757124v1?rss=1
2026-10-07doi:10.64898/2026.10.06.757124Cold Spring Harbor Laboratory2026-10-07
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Structural basis of coiled-coil domain-mediated inhibition of A. baumannii AbTir NAD+ nucleosidase activity
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https://www.biorxiv.org/content/10.64898/2026.10.06.757174v1?rss=1
2026-10-07doi:10.64898/2026.10.06.757174Cold Spring Harbor Laboratory2026-10-07
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Functional characterization of terpene synthases from Dendrobium nobile provides insights into the biosynthesis of copaborneol, a proposed dendrobine precursor
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https://www.biorxiv.org/content/10.64898/2026.10.06.757176v1?rss=1
2026-10-07doi:10.64898/2026.10.06.757176Cold Spring Harbor Laboratory2026-10-07
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The scaffold nucleoporin Nup188 undergoes liquid-liquid phase separation
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https://www.biorxiv.org/content/10.64898/2026.10.06.757041v1?rss=1
2026-10-07doi:10.64898/2026.10.06.757041Cold Spring Harbor Laboratory2026-10-07
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Human FACT complex coordinates cohesin and transcription on chromatin
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https://www.biorxiv.org/content/10.64898/2026.10.06.754922v1?rss=1
2026-10-07doi:10.64898/2026.10.06.754922Cold Spring Harbor Laboratory2026-10-07
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Joining Two Perspectives of the Proteome with the Trypsin+HTA Protease Workflow
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https://www.biorxiv.org/content/10.64898/2026.10.05.756883v1?rss=1
2026-10-06doi:10.64898/2026.10.05.756883Cold Spring Harbor Laboratory2026-10-06
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Peptide frontier-orbital gaps narrow by backbone electrostatics
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https://www.biorxiv.org/content/10.64898/2026.10.05.756722v1?rss=1
-{Delta}(n), whose alignment penalty accounts for the entire effect: the polar backbone generates a monotonic internal electrostatic ramp that leaves the two gap edges at opposite ends of the chain, 32 [A] apart. We set out the resulting design rules for peptide-based bio-organic semiconductors.
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Substrate specificity in lipoylation pathways: How lipoate:protein ligases discriminate between protein substrates
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https://www.biorxiv.org/content/10.64898/2026.10.01.755906v1?rss=1
2026-10-02doi:10.64898/2026.10.01.755906Cold Spring Harbor Laboratory2026-10-02
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A recombinant heterotrimeric mini-procollagen I reveals new functional and structural roles for the α2(I) chain
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https://www.biorxiv.org/content/10.64898/2026.10.03.756409v1?rss=1
2026-10-06doi:10.64898/2026.10.03.756409Cold Spring Harbor Laboratory2026-10-06